Target-induced structure switching of aptamers facilitates strand displacement for DNAzyme recycling amplification detection of thrombin in human serum

The Analyst
Jin LiRuo Yuan

Abstract

To monitor the thrombin concentration under the condition of abnormal blood coagulation is of clinical significance for the diagnosis of various diseases. Here, on the basis of the aptamer structure switching induced by the target molecules and the signal amplification strategy via recycling of metal-ion dependent DNAzymes, we have established a sensitive and simple fluorescent aptasensor for detecting thrombin in human serum. The thrombin target specifically binds to the aptamer sequence and causes a corresponding conformational structure switching, which leads to the formation of a toehold sequence to facilitate the strand migration displacement reaction for the generation of functional metal-ion dependent DNAzymes. These DNAzymes further cleave the fluorescently quenched hairpin substrates cyclically to yield substantially amplified fluorescence recovery for sensitively detecting thrombin in the dynamic range from 0.01 nM to 50 nM. Such an aptasensor shows a detection limit of 6.9 pM and can achieve the monitoring of thrombin in diluted human serum with high selectivity, offering a universal sensing strategy for the construction of various sensitive and simple aptasensors to detect different biomarker molecules.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Nov 16, 2011·Chemical Communications : Chem Comm·Ai-Xian ZhengHuang-Hao Yang
Jan 27, 2012·Chemistry : a European Journal·Xiaoqing LiuItamar Willner
Mar 19, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lingling XieXiaoyu Cao
Jun 4, 2014·Angewandte Chemie·Carl W BrownSteven W Graves
Apr 13, 2015·Talanta·Liping LuShuiyuan Cheng
Nov 10, 2015·Analytica Chimica Acta·Weidan NaXingguang Su
Feb 13, 2016·The Analyst·Muslum Ilgu, Marit Nilsen-Hamilton
Feb 28, 2016·Analytica Chimica Acta·Cristina Ocaña, Manel del Valle
Jun 10, 2017·Chemical Reviews·Wenhu ZhouJuewen Liu
Nov 4, 2017·Analytical Chemistry·Junbo ChenHongquan Zhang
Dec 1, 2017·Analytical Chemistry·Hanyong PengHongquan Zhang
Sep 9, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Wen YunLizhu Yang
Sep 23, 2018·Chemistry : a European Journal·Xiaoxi ZhongRongxing Zhou
Feb 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Juanjuan LiTianming Yao

❮ Previous
Next ❯

Citations

Jul 17, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiqing ZhangFang Wang
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li
Jun 30, 2021·Analytical and Bioanalytical Chemistry·Aida Montserrat PagèsDragana Spasic

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
aptasensors
SELEX
electrophoresis
Fluorescence

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.