PMID: 11914585Mar 27, 2002Paper

Target integration by a chimeric Sp1 zinc finger domain-Moloney murine leukemia virus integrase in vivo

Journal of Biomedical Science
Wen-Jiun PengThy-Hou Lin

Abstract

A specificity protein 1 (Sp1) zinc finger domain containing two tandem zinc fingers was fused to the C terminus of the integrase (IN) protein of the Moloney murine leukemia virus (MuLV). The integrity of the MuLV IN was completely preserved, since the fusion was conducted at the last amino acid residue of the protein. The vector pMIN-Sp1, which carried the fused MuLV IN-Sp1 zinc finger domain gene, was cotransfected with a wild-type MuLV vector pMLV-K to NIH/3T3 cells. A nonradioactive reverse transcriptase assay was performed on culture supernatants collected from the cotransfected cells to confirm the production of recombinant viruses. The expression of the fusion protein and the integration of the MuLV genome by the fusion protein were confirmed by a Northern and then a Southern hybridization analysis on the total RNA or genomic DNA extracted from cells infected by viruses collected from the supernatants of the cotransfected cells. Regions of the host chromosome that were selected by the fusion protein as the integration targets were sequenced using the TOPO(TM) cloning method on a series of PCR products generated with a nested set of primers. The percentage of positive clones screened that contained the DNA-binding sequence...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P M PryciakH E Varmus
Jan 1, 1992·Annual Review of Genetics·S P Goff
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F D Bushman, R Craigie
Feb 1, 1992·AIDS Research and Human Retroviruses·A M WoernerC J Marcus-Sekura
Jun 30, 1992·Biochemical and Biophysical Research Communications·M Schauer, A Billich
Feb 25, 1991·Nucleic Acids Research·E KhanA M Skalka
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M S JohnsonR F Doolittle
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E M StoneR J Schwartz
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y C BorL E Orgel
Jan 1, 1994·Annual Review of Biochemistry·R A Katz, A M Skalka
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P PersengievD L Kilpatrick
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·F D Bushman
Jan 1, 1994·Pharmacology & Therapeutics·J Kulkosky, A M Skalka
Jan 1, 1995·Journal of Virology·I DotanP O Brown
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·D PrussA P Wolffe
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S LeeY Shi
Jul 25, 1993·Nucleic Acids Research·A M Woerner, C J Marcus-Sekura
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R ZhengR Craigie
Feb 1, 1997·Genes & Development·B P ScottolineP O Brown
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Q WeiR Craigie
Jan 13, 2000·Biochemical and Biophysical Research Communications·T MinamitaniK i Matsumoto

❮ Previous
Next ❯

Citations

Feb 3, 2012·Molekuliarnaia biologiia·E S KniazhanskaiaM B Gottikh
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jessica A HurtJ Keith Joung
Mar 9, 2007·Nucleic Acids Research·Stephen R YantMark A Kay
Nov 18, 2005·Advances in Genetics·Mary K Lewinski, Frederic D Bushman
May 2, 2003·Nature Biotechnology·Joseph Kaminski, James Bradley Summers
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kwang-il LimDavid V Schaffer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.