Target irradiation induced bystander effects between stem-like and non stem-like cancer cells

Mutation Research
Yu LiuYugang Wang

Abstract

Tumors are heterogeneous in nature and consist of multiple cell types. Among them, cancer stem-like cells (CSCs) are suggested to be the principal cause of tumor metastasis, resistance and recurrence. Therefore, understanding the behavior of CSCs in direct and indirect irradiations is crucial for clinical radiotherapy. Here, the CSCs and their counterpart non stem-like cancer cells (NSCCs) in human HT1080 fibrosarcoma cell line were sorted and labeled, then the two cell subtypes were mixed together and chosen separately to be irradiated via a proton microbeam. The radiation-induced bystander effect (RIBE) between the CSCs and NSCCs was measured by imaging 53BP1 foci, a widely used indicator for DNA double strand break (DSB). CSCs were found to be less active than NSCCs in both the generation and the response of bystander signals. Moreover, the nitric oxide (NO) scavenger c-PTIO can effectively alleviate the bystander effect in bystander NSCCs but not in bystander CSCs, indicating a difference of the two cell subtypes in NO signal response. To our knowledge, this is the first report shedding light on the RIBE between CSCs and NSCCs, which might contribute to a further understanding of the out-of-field effect in cancer radiotherapy.

References

Mar 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chunlin ShaoKevin M Prise
May 31, 2008·Nature Reviews. Cancer·Michael BaumannRichard Hill
Sep 2, 2008·Cancer Research·Susanne Burdak-RothkammKevin M Prise
Apr 21, 2009·Nature Reviews. Cancer·Kevin M Prise, Joe M O'Sullivan
Dec 15, 2010·Current Molecular Pharmacology·Tom K HeiVladimir N Ivanov
Dec 15, 2010·PloS One·Mykyta V Sokolov, Ronald D Neumann
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios IliopoulosKevin Struhl
Apr 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aroumougame AsaithambyDavid J Chen
Jun 8, 2011·Nature Reviews. Clinical Oncology·Scott C Morgan, Chris C Parker
Dec 16, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jaime Gómez-MillánJosé Mariano Ruiz de Almodóvar
Mar 24, 2012·Cancer Cell·Jeffrey A MageeSean J Morrison
Jan 5, 2013·Journal of Radiation Research·Teruaki KonishiYoshiyuki Shirakawa
Jun 1, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Radosław JanuchowskiMaciej Zabel
Jul 16, 2013·Clinical Oncology : a Journal of the Royal College of Radiologists·M E LomaxP O'Neill
Jul 19, 2013·Physics in Medicine and Biology·Antje-Christin Knopf, Antony Lomax
Nov 12, 2013·Cell·Filippo G Giancotti
Nov 26, 2013·Genome Medicine·Elza C de BruinCharles Swanton
Nov 30, 2013·Nature Reviews. Drug Discovery·Chiara GorriniTak W Mak
Jan 21, 2014·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Amar DesaiStanton L Gerson
Mar 13, 2014·Cell Stem Cell·Thordur OskarssonJoan Massagué

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Citations

Feb 27, 2016·Journal of Cellular Biochemistry·Seog-Young KimYong J Lee
Oct 31, 2018·International Journal of Radiation Biology·Qibin FuTuchen Huang
Oct 26, 2016·Molecular Medicine Reports·Igor S BryukhovetskiyYuri S Khotimchenko

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