Target of rapamycin-mediated amino acid signaling in mosquito anautogeny

Proceedings of the National Academy of Sciences of the United States of America
Immo A HansenAlexander S Raikhel

Abstract

Mosquitoes generate an enormous burden on human health worldwide. Disease-transmitting species use a reproductive strategy, termed anautogeny, that requires a blood meal to initiate egg maturation. Whereas this strategy is important for driving disease transmission, the molecular mechanisms underlying this phenomenon are still poorly understood. The production of yolk protein precursors (YPPs), a central event in egg maturation, is called vitellogenesis. YPPs are synthesized in the fat body, the insect analogue of the vertebrate liver. Mosquito vitellogenesis is regulated by the steroid hormone 20 hydroxyecdysone (20E). However, 20E alone is not capable of activating vitellogenesis in vivo. Here, we report that amino acid signaling through the nutrient-sensitive target of rapamycin (TOR) pathway is essential for the activation of YPP gene expression. An increase in extracellular amino acid levels, similar to the increase observed after a blood meal, is critical for 20E stimulation of YPP gene expression. Treatment with the TOR kinase inhibitor rapamycin significantly inhibits YPP expression. We used RNA interference to knockdown the expression of two key proteins of the TOR signaling pathway, TOR, and tuberous sclerosis complex...Continue Reading

References

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Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Geoffrey M AttardoAlexander S Raikhel

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Citations

Dec 1, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bart BryantAlexander S Raikhel
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tinsley H Davis
Jan 26, 2011·The Journal of Biological Chemistry·Immo A HansenAlexander S Raikhel
Dec 14, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Saurabh G Roy, Alexander S Raikhel
Sep 10, 2004·Science of Aging Knowledge Environment : SAGE KE·Pankaj Kapahi, Brian Zid
Oct 4, 2006·Molecular and Cellular Biology·Jinsong ZhuAlexander S Raikhel
Jul 25, 2012·Journal of Visualized Experiments : JoVE·Lisa L DrakeImmo A Hansen
Aug 10, 2010·Annual Review of Entomology·Daniel A Hahn, David L Denlinger
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·GuoQiang SunAlexander S Raikhel
Dec 4, 2012·Insect Biochemistry and Molecular Biology·Mazhar HussainSassan Asgari
Feb 4, 2011·Insect Biochemistry and Molecular Biology·R Parthasarathy, Subba R Palli
Dec 9, 2015·Current Opinion in Insect Science·Vlastimil Smykal, Alexander S Raikhel
Aug 19, 2008·Insect Biochemistry and Molecular Biology·Michelle C BrandonRoger L Miesfeld
Nov 6, 2007·Journal of Insect Physiology·Shin-Hong ShiaoAlexander S Raikhel
Aug 8, 2007·Insect Biochemistry and Molecular Biology·Immo A HansenAlexander S Raikhel
Dec 18, 2013·Microbial Ecology·Eric P CaragataElizabeth A McGraw
May 30, 2015·Scientific Reports·Yifan ZhaiWenqing Zhang
Jan 30, 2007·Insect Molecular Biology·J IsoeR L Miesfeld

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