Target-programmed and autonomous proximity binding aptasensor for amplified electronic detection of thrombin

Biosensors & Bioelectronics
Jianmei YangYun Xiang

Abstract

The development of sensitive and simple approaches capable of monitoring trace amounts of protein biomarkers is appealing for disease diagnosis and treatment. Towards this end, we have developed an electrochemical sensing platform for sensitive and simple detection of protein biomarkers by using thrombin as the model target molecules via a target-programmed proximity binding amplification approach. The binding of thrombin to the aptamer sequences in the partial dsDNA duplex probes induces the release of the ssDNA trigger strands, which catalyze subsequent assembly formation of many methylene blue (MB)-tagged proximate DNA motifs with the presence of the DNA fuel strands through cascaded toehold-mediated strand displacement reactions. Due to the proximity-binding effect, these MB-tagged proximate DNA motifs anneal with the capture probes on the sensor surface with significantly enhanced stability against the corresponding single component counterpart, thereby pulling the MB tags close to the sensor surface and generating substantially amplified signal responses for sensitive determination of thrombin down to 23.6 pM. In addition, such aptasensor can specifically discriminate thrombin from other interference proteins, and can als...Continue Reading

Citations

Jul 31, 2020·Analytical Methods : Advancing Methods and Applications·Ying ChenXia Zuo
Apr 22, 2019·Analytica Chimica Acta·Morteza Alinezhad NameghiSeyed Mohammad Taghdisi
Nov 5, 2020·Analytical Chemistry·Andresa B BezerraChristopher J Easley

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