Target-regulated proximity hybridization with three-way DNA junction for in situ enhanced electronic detection of marine biotoxin based on isothermal cycling signal amplification strategy

Biosensors & Bioelectronics
Bingqian LiuDianping Tang

Abstract

A new signal amplification strategy based on target-regulated DNA proximity hybridization (TRPH) reaction accompanying formation of three-way DNA junction was designed for electronic detection of Microcystin-LR (MC-LR used in this case), coupling with junction-induced isothermal cycling signal amplification. Initially, a sandwiched-type immunoreaction was carried out in a low-cost PCR tube between anti-MC-LR mAb1 antibody-labeled DNA1 (mAb1-DNA1) and anti-MC-LR mAb2-labeled DNA2 (mAb2-DNA2) in the presence of target to form a three-way DNA junction. Then, the junction could undergo an unbiased strand displacement reaction on an h-like DNA nanostructure-modified electrode (labeled with methylene blue redox tag on the short DNA strand), thereby resulting in the dissociation of methylene blue-labeled signal DNA from the electrode. The newly formed double-stranded DNA could be cleaved again by exonuclease III, and the released three-way DNA junction retriggered the strand-displacement reaction with h-like DNA nanostructures for junction recycling. During the strand-displacement reaction, numerous methylene blue-labeled DNA strands were far away from the electrode, thus decreasing the detectable electrochemical signal within the app...Continue Reading

References

Dec 22, 1986·Nucleic Acids Research·R I MaN C Seeman
Oct 5, 2010·Chemical Communications : Chem Comm·Lei YanHerman O Sintim

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Citations

Jun 9, 2015·Chemical Communications : Chem Comm·Jing LiuShusheng Zhang
Feb 24, 2016·The Analyst·Xiaolong YangFeng Li
Dec 9, 2020·Critical Reviews in Food Science and Nutrition·Qi WangWei Wu
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin

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