Target selectivity of septal cholinergic neurons in the medial and lateral entorhinal cortex

Proceedings of the National Academy of Sciences of the United States of America
Srinidhi DesikanHannah Monyer

Abstract

The entorhinal cortex (EC) plays a pivotal role in processing and conveying spatial information to the hippocampus. It has long been known that EC neurons are modulated by cholinergic input from the medial septum. However, little is known as to how synaptic release of acetylcholine affects the different cell types in EC. Here we combined optogenetics and patch-clamp recordings to study the effect of cholinergic axon stimulation on distinct neurons in EC. We found dense cholinergic innervations that terminate in layer I and II (LI and LII). Light-activated stimulation of septal cholinergic projections revealed differential responses in excitatory and inhibitory neurons in LI and LII of both medial and lateral EC. We observed depolarizing responses mediated by nicotinic and muscarinic receptors primarily in putative serotonin receptor (p5HT3R)-expressing interneurons. Hyperpolarizing muscarinic receptor-mediated responses were found predominantly in excitatory cells. Additionally, some excitatory as well as a higher fraction of inhibitory neurons received mono- and/or polysynaptic GABAergic inputs, revealing that medial septum cholinergic neurons have the capacity to corelease GABA alongside acetylcholine. Notably, the synaptic e...Continue Reading

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Citations

Jul 12, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tao JinFeifei Wang
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Sep 26, 2019·Frontiers in Molecular Neuroscience·Noorya Yasmin AhmedNathalie Dehorter
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Jan 28, 2021·Brain : a Journal of Neurology·Yuichi TakeuchiAntal Berényi
Jan 7, 2021·International Journal of Molecular Sciences·Kazuya MatsuoKohji Fukunaga
Mar 23, 2021·Frontiers in Cellular Neuroscience·Angel Nuñez, Washington Buño
Jul 27, 2021·Frontiers in Neural Circuits·Yuichi TakeuchiAntal Berényi
Aug 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·James DelormeSara J Aton

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

MATLAB
ImageJ

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