PMID: 8603079Mar 13, 1996Paper

Target-sensitive immunoerythrocytes: interaction of biotinylated red blood cells with immobilized avidin induces their lysis by complement

Biochimica Et Biophysica Acta
V R MuzykantovB P Morgan

Abstract

Red blood cells (RBC) coated with antibody (immunoerythrocytes) may be useful for drug targeting. Previously we have developed a methodology for avidin (streptavidin)-mediated attachment of biotinylated antibodies (b-Ab) to biotinylated RBC (B-RBC). We have observed that binding of avidin to B-RBC in suspension leads to their complement-mediated lysis by autologous serum. In the present work we have studied the interaction of B-RBC, which are not complement susceptible, with immobilized avidin and their consequent susceptibility to lysis by complement. B-RBC adhered tightly to avidin-coated surfaces and were rendered susceptible to lysis by autologous serum. A long biotin ester provided more effective binding of the B-RBC to immobilized avidin and greater lysis by complement, than a short biotin ester. Based on these results, we have hypothesized that targeting of serum-stable drug-loaded B-RBC attained by step-wise administration of b-Ab and streptavidin may provide target-sensitive lysis of B-RBC. To confirm this hypothesis, we have studied b-Ab and streptavidin mediated targeting of B-RBC to immobilized antigen. Step-wise addition of biotinylated antibody, avidin or streptavidin and b-RBC caused specific binding of B-RBC to ...Continue Reading

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Citations

Aug 1, 2015·Therapeutic Delivery·Carlos H VillaVladimir R Muzykantov
Oct 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yanan SunMingfeng Qiu
Oct 7, 2016·Transfusion Medicine Reviews·Carlos H VillaVladimir Muzykantov
Mar 3, 2010·Expert Opinion on Drug Delivery·Vladimir R Muzykantov
Mar 22, 2020·Pharmaceutics·Larisa KolevaElena Sinauridze
Dec 3, 2020·Biosensors & Bioelectronics·Ruixue ZhuIgor Meglinski
Mar 17, 2021·European Journal of Pharmacology·Nur Najihah Izzati Mat RaniMohd Cairul Iqbal Mohd Amin

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