Target site insensitivity mutations in the AChE enzyme confer resistance to organophosphorous insecticides in Leptinotarsa decemlineata (Say)

Pesticide Biochemistry and Physiology
M Malekmohammadi, H Galehdari

Abstract

In the present study, we demonstrated the use and optimization of the tetra-primer ARMS-PCR procedure to detect and analyze the frequency of the R30K and I392T mutations in resistant field populations of CPB. The R30K mutation was detected in 72%, 84%, 52% and 64% of Bahar, Dehpiaz, Aliabad and Yengijeh populations, respectively. Overall frequencies of the I392T mutation were 12%, 8% and 16% of Bahar, Aliabad and Yengijeh populations, respectively. No I392T point mutation was found among samples from Dehpiaz field population. Moreover, only 31% and 2% of samples from the resistant field populations were homozygous for R30K and I392T mutations, respectively. No individual simultaneously had both I392T and S291G/R30K point mutations. The incidence of individuals with both S291G and R30K point mutations in the samples from Bahar, Dehpiaz, Aliabad, and Yengijeh populations were 31.5%, 44.7%, 41.6%, and 27.3% respectively. Genotypes determined by the tetra-primer ARMS-PCR method were consistent with those determined by PCR sequencing. There was no significant correlation between the mutation frequencies and resistance levels in the resistant populations, indicating that other mutations may contribute to this variation. Polymorphism ...Continue Reading

References

Aug 28, 2001·Nucleic Acids Research·S YeI N Day
Apr 3, 2004·Genome Génome / Conseil National De Recherches Canada·E ChiapparinoP Donini
Dec 13, 2005·Journal of Economic Entomology·G Christopher CutlerC Ronald Harris
Jun 22, 2011·Insect Biochemistry and Molecular Biology·L RevueltaP Hernández-Crespo

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Citations

Jul 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiangkun MengZewen Liu
Oct 29, 2021·Environmental Science and Pollution Research International·Muhammad HafeezYaobin Lu

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