Target-triggered configuration change of DNA tetrahedron for SERS assay of microRNA 122.

Mikrochimica Acta
Shufan WangXia Yang

Abstract

A surface-enhanced Raman scattering (SERS) method is proposed for the assay of microRNA 122 based on configuration change of DNA tetrahedron. Firstly, a DNA tetrahedron was self-assembled with one vertex labeled with toluidine blue (TB). Then, it was immobilized on the porous Ni/SiO2@PEI@Au as a SERS platform, which was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). At this time, the DNA tetrahedron was contracted; so, the TB is close to AuNPs and the Raman signal is high. When target microRNA 122 existed, with the nicking enzyme amplification strategy, a great deal of DNA signal chains (S5) was obtained, which can extend the contracted DNA tetrahedron and change it into a three-dimensional DNA tetrahedron. In this case, the TB was far from AuNPs, resulting in a lower Raman signal. Due to the configuration change of DNA tetrahedron, the Raman signal at 1624 cm-1 (with the excitation wavelength of 633 nm) has a linear relationship with the logarithm concentration of microRNA 122. This SERS assay has high sensitivity for microRNA 122 with a determination range from 0.01 aM to 10 fM and a detection limit of 0.009 aM. The recoveries from spiked samples were in the range 95 to 109%. This SERS strate...Continue Reading

References

Mar 24, 2018·Cell·David P Bartel
Jun 30, 2019·Analytical Biochemistry·Zahra ShabaninejadHamed Mirzaei
Aug 6, 2019·Biosensors & Bioelectronics·Zhipeng HuangJilie Kong
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yi HeYaqin Chai

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Citations

Jun 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jingcheng CuiGuiqin Zhang

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