Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Timothy SibandaMemory Tekere

Abstract

This study aimed to analyze the bacterial diversity in carwash effluents and to determine their potential for use in microbial degradation of environmental contaminants. Nine carwash effluent samples were collected for physicochemical and bacterial community diversity analysis using multi-digital probes and 16S rRNA gene amplicon sequencing respectively. The pH of all effluent samples was neutral to slightly alkaline. Oil and grease concentrations ranged from 15.3 to 49.7 mg/L. 16S gene amplicon sequencing of the nine samples produced 45,934-sequence reads, which translated to 13 bacterial phyla, 26 classes, and 43 genera. The most dominant phyla were Proteobacteria, Bacteroidetes, Firmicutes, and Fusobacteria. Canonical correspondence analysis (CCA) showed that the distribution of the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Verrucomicrobia was influenced by the presence of oil and grease, total petroleum hydrocarbons-gasoline range organics (GRO-TPH), and metals species (Pb, Cu, and Zn). The dominant bacterial genera found in the present study were previously proven to biodegrade hydrocarbons, and their presence in carwash effluents could bode well for in situ natural bioremediation of these contaminated sites.

References

Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Catherine A Lozupone, Rob Knight
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
May 5, 2012·Bioinformatics·Elmar PruesseFrank Oliver Glöckner
Sep 15, 2012·The ISME Journal·Viggó Thór MarteinssonEric Gaidos
Nov 13, 2012·Applied and Environmental Microbiology·Nora B SuttonHauke Smidt
Nov 23, 2012·Environmental Microbiology·Gilberto E FloresNoah Fierer
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Oct 1, 2013·Applied and Environmental Microbiology·Maria C PortilloNoah Fierer
Jan 10, 2014·Microbial Ecology·Marcin GołębiewskiBorys Wróbel
Apr 2, 2014·Environmental Pollution·Steven WakelinMaureen O'Callaghan
Nov 25, 2014·Applied Microbiology and Biotechnology·Jitendra KeshriMaggy N B Momba
Sep 26, 2015·International Journal of Environmental Research and Public Health·Mu PengQiuyu Wang
Mar 10, 2016·Environmental Science and Pollution Research International·Memory TekereKhumbudzo Walter Maphangwa
Jun 2, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Obinna C NwinyiOlukayode O Amund
Mar 31, 2017·Scientific Reports·P LaiY Zhou
Apr 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ramganesh SelvarajanStephen Meddows-Taylor

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP117897

Methods Mentioned

BETA
amplicon sequencing
RSA
PCR

Software Mentioned

SINA SINA
SINA
SASOL
XLSTAT
PAST
SILVA Incremental Aligner (

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.