Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques.

Analytical and Bioanalytical Chemistry
Federica Dal BelloClaudio Medana

Abstract

Quorum sensing (QS) is the ability of some bacteria to detect and to respond to population density through signalling molecules. QS molecules are involved in motility and cell aggregation mechanisms in diseases such as sepsis. Few biomarkers are currently available to diagnose sepsis, especially in high-risk conditions. The aim of this study was the development of new analytical methods based on liquid chromatography-mass spectrometry for the detection and quantification of QS signalling molecules, including N-acyl homoserine lactones (AHL) and hydroxyquinolones (HQ), in biofluids. Biological samples used in the study were Pseudomonas aeruginosa bacterial cultures and plasma from patients with sepsis. We developed two MS analytical methods, based on neutral loss (NL) and product ion (PI) experiments, to identify and characterize unknown AHL and HQ molecules. We then established a multiple-reaction-monitoring (MRM) method to quantify specific QS compounds. We validated the HPLC-MS-based approaches (MRM-NL-PI), and data were in accord with the validation guidelines. With the NL and PI MS-based methods, we identified and characterized 3 and 13 unknown AHL and HQ compounds, respectively, in biological samples. One of the newly foun...Continue Reading

References

Aug 19, 2000·Infection and Immunity·T R de Kievit, B H Iglewski
Mar 12, 2002·FEMS Microbiology Letters·Barry MiddletonAndrea Hardman
Jul 29, 2006·Environmental Microbiology·Florian BredenbruchSusanne Häussler
Jan 20, 2007·FEMS Microbiology Letters·Laura Steindler, Vittorio Venturi
Sep 18, 2007·Advances in Applied Microbiology·Yevgeniy TurovskiyMichael L Chikindas
Nov 16, 2007·Nature·Stephen P DiggleStuart A West
Apr 15, 2008·Analytical and Bioanalytical Chemistry·Anjali KumariSylvia Daunert
Feb 25, 2010·Natural Product Reports·Jeroen S Dickschat
Sep 19, 2012·Analytical and Bioanalytical Chemistry·Tommaso R I CataldiPhilippe Schmitt-Kopplin
Nov 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anand PaiLingchong You
Feb 20, 2015·Microbiology and Molecular Biology Reviews : MMBR·Burkhard A Hense, Martin Schuster
Feb 6, 2017·Journal of Analytical Toxicology·Brigitte DesharnaisCameron D Skinner
Sep 14, 2017·Chemical Society Reviews·D LeichnitzC Beemelmanns
Dec 17, 2017·Chemico-biological Interactions·Diby PaulMuthu Manikandan
May 24, 2018·MBio·Rhea G AbisadoJosephine R Chandler
Jul 20, 2018·Frontiers in Cellular and Infection Microbiology·Jinshui LinXihui Shen
Apr 5, 2019·Nature Reviews. Microbiology·Sampriti Mukherjee, Bonnie L Bassler
Dec 4, 2019·Journal of Medical Microbiology·Selene García-ReyesMiguel Cocotl-Yanez
Feb 1, 2020·Trends in Microbiology·Rebecca D Prescott, Alan W Decho
Mar 21, 2020·Analytical and Bioanalytical Chemistry·Elizabeth ReadelDaniel W Armstrong
Mar 25, 2020·JAMA : the Journal of the American Medical Association·Jean-Louis VincentUNKNOWN EPIC III Investigators

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Methods Mentioned

BETA
biosensors
NMR

Software Mentioned

NL
PI
LTQ

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