Targeted-bisulfite sequence analysis of the methylation of CpG islands in genes encoding PNPLA3, SAMM50, and PARVB of patients with non-alcoholic fatty liver disease

Journal of Hepatology
Takuya KitamotoKikuko Hotta

Abstract

The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is affected by epigenetic factors as well as by genetic variation. We performed targeted-bisulfite sequencing to determine the levels of DNA methylation of 4 CpG islands (CpG99, CpG71, CpG26, and CpG101) in the regulatory regions of PNPLA3, SAMM50, PARVB variant 1, and PARVB variant 2, respectively. We compared the levels of methylation of DNA in the livers of the first and second sets of patients with mild (fibrosis stages 0 and 1) or advanced (fibrosis stages 2 to 4) NAFLD and in those of patients with mild (F0 to F2) or advanced (F3 and F4) chronic hepatitis C infection. The hepatic mRNA levels of PNPLA3, SAMM50, and PARVB were measured using qPCR. CpG26, which resides in the regulatory region of PARVB variant 1, was markedly hypomethylated in the livers of patients with advanced NAFLD. Conversely, CpG99 in the regulatory region of PNPLA3 was substantially hypermethylated in these patients. These differences in DNA methylation were replicated in a second set of patients with NAFLD or chronic hepatitis C. PNPLA3 mRNA levels in the liver of the same section of a biopsy specimen used for genomic DNA preparation were lower in patients with advanced NAFLD compared with...Continue Reading

References

Dec 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M R TeliC P Day
May 29, 1999·Gastroenterology·C A MatteoniA J McCullough
Apr 12, 2001·Seminars in Liver Disease·E M Brunt
Apr 19, 2002·The New England Journal of Medicine·Paul Angulo
Nov 7, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·UNKNOWN Examination Committee of Criteria for 'Obesity Disease' in Japan, UNKNOWN Japan Society for the Study of Obesity
Jan 25, 2003·Journal of Gastroenterology and Hepatology·Geoffrey C Farrell
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
May 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David E KleinerUNKNOWN Nonalcoholic Steatohepatitis Clinical Research Network
Aug 16, 2006·Journal of Atherosclerosis and Thrombosis·Hidenori AraiToru Kita
May 1, 2008·Current Diabetes Reports·Nimantha Mark Wilfred de Alwis, Christopher Paul Day
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Aug 17, 2010·Gastroenterology·Naga ChalasaniUNKNOWN Nonalcoholic Steatohepatitis Clinical Research Network
Oct 5, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Silvia SookoianCarlos J Pirola
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Takeshi OkanoueYoshito Itoh
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Mar 8, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Silvia Sookoian, Carlos J Pirola
Apr 16, 2011·Bioinformatics·Felix Krueger, Simon R Andrews
Jan 19, 2012·Endocrinology·Yoshinori SekiMaureen J Charron
Feb 1, 2012·Nature Methods·Felix KruegerSimon R Andrews
Feb 9, 2012·Journal of Gastroenterology·Hideki Fujii, Norifumi Kawada
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jun 22, 2012·PloS One·Takahisa KawaguchiUNKNOWN Japan Study Group of Nonalcoholic Fatty Liver Disease
Aug 21, 2013·Nature Reviews. Gastroenterology & Hepatology·Katrina Ray
Feb 13, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Dirk S PaulStephan Beck

❮ Previous
Next ❯

Citations

Jan 1, 2015·Nature Reviews. Disease Primers·Elizabeth M BruntMary E Rinella
Oct 18, 2015·Trends in Cardiovascular Medicine·Hui TaoJun Li
Feb 14, 2016·Gastroenterology·Quentin M AnsteeChristopher P Day
Apr 30, 2016·International Journal of Molecular Sciences·Gemma AragonèsCristóbal Richart
Jan 15, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yasushi HondaKikuko Hotta
Feb 22, 2017·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kikuko HottaAtsushi Nakajima
Nov 26, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Benedetta DonatiLuca Valenti
Jan 19, 2019·Clinical Epigenetics·Paula WalleJussi Pihlajamäki
Jun 2, 2020·Expert Review of Gastroenterology & Hepatology·Carlos Jose Pirola, Silvia Sookoian
Jul 7, 2019·Nature Reviews. Gastroenterology & Hepatology·Valerio NobiliNaim Alkhouri
Jul 15, 2018·Clinical Epigenetics·Glenn S GerhardJohanna K DiStefano
Sep 15, 2016·Gut·Timothy Hardy, Derek A Mann
Oct 24, 2017·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kikuko HottaAtsushi Nakajima
Dec 23, 2020·European Journal of Clinical Investigation·Xiaofang ZhangMohsen Ghanbari
Nov 20, 2020·International Journal of Molecular Sciences·Hyun Jung Lim, Mirang Kim
Feb 16, 2021·World Journal of Gastroenterology : WJG·Carlos Jose PirolaSilvia Sookoian
Feb 12, 2020·Gastroenterology·Mohammed EslamUNKNOWN International Consensus Panel
May 6, 2021·American Journal of Medical Genetics. Part a·Arianna RicciardelloAntonio M Persico
May 4, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Anat Yaskolka MeirIris Shai
May 29, 2021·Frontiers in Endocrinology·Hannah MaudeInês Cebola

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.