Targeted chromatin profiling reveals novel enhancers in Ig H and Ig L chain Loci

The Journal of Immunology : Official Journal of the American Association of Immunologists
Alexander V PredeusMaxim N Artyomov

Abstract

The assembly and expression of mouse Ag receptor genes are controlled by a collection of cis-acting regulatory elements, including transcriptional promoters and enhancers. Although many powerful enhancers have been identified for Ig (Ig) and TCR (Tcr) loci, it remained unclear whether additional regulatory elements remain undiscovered. In this study, we use chromatin profiling of pro-B cells to define 38 epigenetic states in mouse Ag receptor loci, each of which reflects a distinct regulatory potential. One of these chromatin states corresponds to known transcriptional enhancers and identifies a new set of candidate elements in all three Ig loci. Four of the candidates were subjected to functional assays, and all four exhibit enhancer activity in B but not in T lineage cells. The new regulatory elements identified by focused chromatin profiling most likely have important functions in the creation, refinement, and expression of Ig repertoires.

References

Aug 31, 2007·Genome Biology·Jun S SongX Shirley Liu
Nov 23, 2007·Nature·Adam G W MatthewsMarjorie A Oettinger
Jan 2, 2009·Advances in Immunology·Thomas Perlot, Frederick W Alt
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
May 30, 2009·Bioinformatics·Thomas AbeelYvan Saeys
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Sep 11, 2010·Seminars in Immunology·Oleg Osipovich, Eugene M Oltz
Sep 14, 2010·Seminars in Immunology·Stephanie C Degner-Leisso, Ann J Feeney
Sep 14, 2010·Current Biology : CB·Oliver Hobert
Mar 1, 2012·Nature Methods·Jason Ernst, Manolis Kellis
Mar 20, 2012·Nature Methods·Michael M HoffmanWilliam Stafford Noble
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jiyoti Verma-GaurAnn J Feeney
Jul 31, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nancy M ChoiAnn J Feeney
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Suhasni GopalakrishnanEugene M Oltz
Nov 19, 2013·Genes & Development·Satyendra KumarAmy L Kenter

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Citations

Apr 3, 2014·PLoS Biology·Jean-Marie BuersteddeDavid G Schatz
Feb 24, 2016·The Journal of Experimental Medicine·Anne-Sophie Thomas-ClaudepierreBernardo Reina-San-Martin
Mar 30, 2017·Scientific Reports·A SaintamandY Denizot
Jul 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joana M SantosAhmed Amine Khamlichi
Dec 13, 2019·Transcription·E Mauricio Barajas-Mora, Ann J Feeney
Aug 7, 2019·Cellular & Molecular Immunology·Rocío Amoretti-VillaBernardo Reina-San-Martin
Sep 6, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna IacoangeliEllen Hsu
Jan 12, 2021·FEBS Open Bio·Faizan Uddin, Madhulika Srivastava

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