Targeted deletion mutagenesis of the beta subunit of cytochrome b559 protein destabilizes the reaction center of photosystem II

Zeitschrift Für Naturforschung. C, a Journal of Biosciences
H B PakrasiH Granok

Abstract

Oligonucleotide-directed mutagenesis techniques were used to delete the psbF gene, encoding the beta subunit of the cytochrome b559 protein of the photosystem II complex in the cyanobacterium, Synechocystis 6803. Cyt b559 is an integral component of PS II complex. However, its precise functional role in PS II remains to be determined. Previously, we created a mutant in which the psbF gene as well as three of its neighbouring genes, psbE, psbL and psbJ were simultaneously deleted from the chromosome of Synechocystis 6803 (Pakrasi, Williams and Arntzen, EMBO J. 7, 325-332, 1988). This mutant had no PS II activity. However, the role of any one of the four individual gene products could not be determined by studying this mutant. The newly generated mutant, T256, had only one gene, psbF, deleted from the genome. This mutant was also impaired in its PS II activities. In addition, it had barely detectable levels of two other protein components, D1 (herbicide binding protein) and D2, of the reaction center of PS II, in its thylakoid membranes. In contrast, two other proteins of PS II, CP47 and CP43 were present in appreciable amounts. Fluorescence spectra (77 K) of the mutant showed the absence of a peak at 695 nm that was previously b...Continue Reading

Citations

Aug 27, 2003·The Journal of Biological Chemistry·Ikuyo Sugimoto, Yuichiro Takahashi
Feb 19, 2013·Photosynthesis Research·Cristina PaglianoJames Barber
Nov 20, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elena Baena-González, Eva-Mari Aro
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·K H Rhee
Sep 22, 2007·Journal of Bioenergetics and Biomembranes·Arjun TiwariSudhakar Bharti
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai
Oct 24, 1998·Biochimica Et Biophysica Acta·D H Stewart, G W Brudvig
Feb 12, 2004·Biochimica Et Biophysica Acta·Lan-Xin Shi, Wolfgang P Schröder
Sep 13, 2011·Biochimica Et Biophysica Acta·Lan-Xin ShiWolfgang P Schröder

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