Targeted deletion of miR-132/-212 impairs memory and alters the hippocampal transcriptome

Learning & Memory
Katelin F HansenKarl Obrietan

Abstract

miR-132 and miR-212 are structurally related microRNAs that have been found to exert powerful modulatory effects within the central nervous system (CNS). Notably, these microRNAs are tandomly processed from the same noncoding transcript, and share a common seed sequence: thus it has been difficult to assess the distinct contribution of each microRNA to gene expression within the CNS. Here, we employed a combination of conditional knockout and transgenic mouse models to examine the contribution of the miR-132/-212 gene locus to learning and memory, and then to assess the distinct effects that each microRNA has on hippocampal gene expression. Using a conditional deletion approach, we show that miR-132/-212 double-knockout mice exhibit significant cognitive deficits in spatial memory, recognition memory, and in tests of novel object recognition. Next, we utilized transgenic miR-132 and miR-212 overexpression mouse lines and the miR-132/-212 double-knockout line to explore the distinct effects of these two miRNAs on the transcriptional profile of the hippocampus. Illumina sequencing revealed that miR-132/-212 deletion increased the expression of 1138 genes; Venn analysis showed that 96 of these genes were also downregulated in mice...Continue Reading

References

Mar 25, 1993·Nature·T SöllnerJ E Rothman
Dec 1, 1996·The European Journal of Neuroscience·B Ruiz-MontasellJ Blasi
Apr 12, 2001·The European Journal of Neuroscience·J HallB J Everitt
May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
Jul 12, 2002·Behavioural Brain Research·Makoto MizunoToshitaka Nabeshima
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·John KimGary Ruvkun
Oct 22, 2004·The EMBO Journal·Carlos M ParrasFrançois Guillemot
Feb 22, 2005·Neuropharmacology·Simon J Mitchell, Timothy A Ryan
Mar 19, 2005·Science·Antonio J GiraldezAlexander F Schier
Sep 16, 2005·Molecular and Cellular Neurosciences·Leigh Anne SwayneJanice E A Braun
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ngan VoSoren Impey
Jan 20, 2006·Nature·Gerhard M SchrattMichael E Greenberg
Jun 16, 2007·Nature Protocols·Gregor ObernostererMattias Alenius
Nov 13, 2007·Nature Neuroscience·Matthew E KleinRichard H Goodman
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Euiseok J KimJane E Johnson
Dec 18, 2007·Neurobiology of Disease·Rory JohnsonNoel J Buckley
Dec 26, 2007·Nucleic Acids Research·Doron BetelChris Sander
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Trinna L CuellarMichael T McManus
Apr 23, 2008·Current Protocols in Neuroscience·Jeanne M Wehner, Richard A Radcliffe
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tigwa H DavisErik M Ullian
Jun 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gary A WaymanSoren Impey
Jul 3, 2008·The International Journal of Neuropsychopharmacology·Kazuhiko NakamuraNorio Mori
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jan 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amy N PackerBeverly L Davidson
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Oct 6, 2009·The International Journal of Biochemistry & Cell Biology·Kimberly Breving, Aurora Esquela-Kerscher
Oct 24, 2009·Molecular and Cellular Neurosciences·Soren ImpeyGary A Wayman
Dec 23, 2009·Molecular Neurodegeneration·Se Hoon ChoiSangram S Sisodia
Jun 30, 2010·Molecular and Cellular Biology·Kotb AbdelmohsenMyriam Gorospe
Jul 9, 2010·Nature·Jonathan A HollanderPaul J Kenny

❮ Previous
Next ❯

Citations

Apr 28, 2016·Progress in Neuro-psychopharmacology & Biological Psychiatry·Julia Hernandez-RappSébastien S Hébert
Dec 17, 2016·Molecular Neurobiology·Na AnHao Yang
May 24, 2017·Nature Communications·Raffaele MazziottiTommaso Pizzorusso
Mar 13, 2018·CNS Neuroscience & Therapeutics·Ting Cao, Xue-Chu Zhen
Aug 24, 2017·International Journal of Molecular Sciences·Nadine PaeschkeClaudia D Spies
Mar 3, 2018·International Journal of Molecular Sciences·Nan MiaoTao Sun
Feb 26, 2019·Genes, Brain, and Behavior·Li-Ching LeeChun-Yen Chang
Aug 24, 2019·Current Neurology and Neuroscience Reports·Mary XylakiTiago Fleming Outeiro
Dec 5, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·Stylianos RavanidisEpaminondas Doxakis
Aug 25, 2018·Annals of Neurosciences·Subramanian ThangaleelaKoilmani Emmanuvel Rajan
Jun 18, 2020·Nature Reviews. Neurology·Gary P Brennan, David C Henshall
Jun 27, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenifer C Kaldun, Simon G Sprecher
May 5, 2020·Frontiers in Neurology·Xianjuan KouNing Chen
Jan 27, 2019·Scientific Reports·Marianne RonovskyDaniela D Pollak
Oct 24, 2018·Frontiers in Neuroscience·Shinya TasakiDavid A Bennett
Jun 2, 2018·Scientific Reports·A HadarD Gurwitz
Dec 12, 2020·Journal of Cellular Physiology·Hanane Hadj-MoussaKenneth B Storey
Dec 16, 2019·Neurochemistry International·Mario J BertogliatRaghu Vemuganti
Feb 13, 2021·Biomolecules·Matea Nikolac PerkovicNela Pivac
May 5, 2021·Translational Psychiatry·Helena Caria Martins, Gerhard Schratt
Mar 24, 2018·Cell·David P Bartel
May 23, 2021·Advances in Nutrition·Christine LerouxJ Bruce German

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.