PMID: 7524726Oct 1, 1994Paper

Targeted deletion of psaJ from the cyanobacterium Synechocystis sp. PCC 6803 indicates structural interactions between the PsaJ and PsaF subunits of photosystem I

Plant Molecular Biology
Q XuP R Chitnis

Abstract

Photosystem I catalyzes the light-driven oxidation of plastocyanin or cytochrome c6 and the reduction of ferredoxin or flavodoxin. PsaJ is a 4.4 kDa hydrophobic subunit of photosystem I from cyanobacteria and chloroplasts. To investigate the function of PsaJ, we generated a mutant strain of the cyanobacterium Synechocystis sp. PCC 6803 in which the psaJ gene is replaced by a gene for chloramphenicol resistance. Deletion of psaJ led to a reduction in the steady state RNA level from psaF which is located upstream from psaJ. Immunoquantification using an anti-PsaF antibody revealed a significant decrease in the amount of PsaF in membranes of the mutant strain. Trimeric photosystem I complexes isolated from the mutant strain using n-dodecyl beta-D-maltoside lacked PsaJ, contained ca. 80% less PsaF, but maintained wild-type levels of other photosystem I subunits. In contrast, the photosystem I purified using Triton X-100 contained less than 2% PsaF when compared to the wild type, showing the more extractable nature of PsaF in PsaJ-less photosystem I in the presence of Triton X-100. PsaE was more accessible to removal by NaI in a mutant strain lacking PsaF and PsaJ than in the wild type. The presence of PsaF in photosystem I from the...Continue Reading

References

Jul 5, 1993·Biochimica Et Biophysica Acta·N Weber, H Strotmann
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J H Golbeck

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Citations

May 1, 1995·Photosynthesis Research·P R ChitnisR Nechushtai
Jun 25, 2005·Photosynthesis Research·Ingo Grotjohann, Petra Fromme
Mar 17, 1999·Biochimica Et Biophysica Acta·P JinP R Chitnis
Apr 28, 2007·Proteomics·Lello ZollaAnna Maria Timperio
Aug 29, 1997·The Journal of Biological Chemistry·J SunP R Chitnis
Nov 1, 2001·Biochimica Et Biophysica Acta·W XuP R Chitnis
Nov 1, 2001·Biochimica Et Biophysica Acta·H V SchellerJ Knoetzel
Nov 1, 2001·Biochimica Et Biophysica Acta·P FrommeN Krauss
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai
May 15, 2021·Current Biology : CB·Nasim RahmatpourFay-Wei Li

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