Targeted delivery of adenosine 5'-triphosphate using chitosan-coated mesoporous hydroxyapatite: A theranostic pH-sensitive nanoplatform with enhanced anti-cancer effect

International Journal of Biological Macromolecules
Mohadeseh S Feiz, Azadeh Meshkini

Abstract

In this Study, a pH-sensitive nanoplatform made up of chitosan (Cs) and mesoporous hydroxyapatite (HAP) was synthesized and employed for delivering of adenosine 5'-triphosphate (ATP). The fabricated system was decorated with folic acid (FA), providing both tumor targeting and imaging. The FA.Cs.ATP@HAP nanoparticles displayed enhanced colloidal stability and controlled drug release. In vitro biological experiments revealed that FA.Cs.ATP@HAP was internalized into the tumor cells with a high efficiency in a time-dependent manner and exhibited strong fluorescence within the cells. Compared with free ATP, the FA.Cs.ATP@HAP nanoparticles exhibited a significant inhibition effect against the proliferation of the tumor cells (Saos-2, T47D, and MCF7) in a dose-dependent manner, while no significant cytotoxic effect was observed in the normal cells (HEK-293), indicating the selective cytotoxicity of the fabricated nanosystem against the tumor cells. Also, the mechanism of action of FA.Cs.ATP@HAP was investigated, and it was found that it induces a high rate of apoptosis in the tumor cells through a decrease in mitochondrial membrane potential and caspase activation. Based on these findings, FA.Cs.ATP@HAP is a novel biomedical material ...Continue Reading

Citations

Jan 26, 2020·Nanomaterials·Ylenia JabaleraJaime Gómez-Morales
Nov 21, 2020·Cells·Valentina Vultaggio-PomaFrancesco Di Virgilio
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mural QuadrosGunjan Verma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Cell Invasion: Nanomedicine

Nanomedicine is a promising alternative for cancer detection and therapy that utilizes nanoparticles, such as liposomes. Nanoparticles can potentially target cancer cell invasion and metastasis. Discover the latest research on Cancer Cell Invasion: Nanomedicine here.