Targeted Delivery of an Activatable Fluorescent Probe for the Detection of Furin Activity in Living Cells

Chembiochem : a European Journal of Chemical Biology
Xueyu ZhaoJianguo Lin

Abstract

Furin, a protein convertase, plays a crucial role in the progression of tumors. In this work, a new fluorescent probe consisting of a peptide, Arg-Val-Arg-Arg (RVRR), and an aminoluciferin fluorophore was designed and prepared for the responsive and activatable detection of furin activity in vitro and in living cells. We demonstrated that this probe could be responsive toward furin with an "off-on" florescence signal and generated an approximately 3.58-fold enhancement in the fluorescence intensity in vitro. Fluorescence imaging in MDA-MB-468 and LoVo cells showed that the probe could be cleaved by overexpressed furin with fluorescence turn-on in MDA-MB-468 cells, and this probe was also found to be capable of discriminating between furin-overexpressing and furin-deficient cell lines. Our research indicates that this probe has great potential for the detection of furin activity in living cells.

References

Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
May 15, 2008·Bioconjugate Chemistry·Hiroshi AbeYoshihiro Ito
Nov 26, 2008·Nature Medicine·Yasuteru UranoHisataka Kobayashi
Aug 1, 2009·Bioconjugate Chemistry·Anca Dragulescu-AndrasiJianghong Rao
Apr 24, 2010·Angewandte Chemie·Gil G WestmeyerAlan Jasanoff
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jia GuoEric T Kool
Feb 26, 2011·Angewandte Chemie·Deju YeJianghong Rao
May 10, 2011·Chemical Society Reviews·Andrew RazgulinJianghong Rao
Jul 5, 2011·Biomaterials·Shenglin LuoChunmeng Shi
Aug 24, 2012·Chemical Communications : Chem Comm·Qingqing MiaoGaolin Liang
Dec 12, 2012·Journal of the American Chemical Society·Laura E EdgingtonMatthew Bogyo
Jul 19, 2013·Journal of the American Chemical Society·Anca Dragulescu-AndrasiSanjiv S Gambhir
Dec 18, 2013·Organic & Biomolecular Chemistry·Yue Yuan, Gaolin Liang
Nov 12, 2016·Biosensors & Bioelectronics·Wei-Wei ZhaoHong-Yuan Chen
Dec 22, 2016·Chemical Communications : Chem Comm·Xiaomei Liu, Gaolin Liang
Feb 15, 2017·Analytical Chemistry·Yuqi WangHong-Yuan Chen
Jun 14, 2017·Analytical Chemistry·Zijuan HaiGaolin Liang
Jul 12, 2017·ACS Applied Materials & Interfaces·Yizhong ShenHong-Yuan Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.