Targeted delivery of ginsenoside compound K using TPGS/PEG-PCL mixed micelles for effective treatment of lung cancer

International Journal of Nanomedicine
Lei YangHuixia Lv

Abstract

Ginsenoside compound K (CK) is one of the effective ingredients in antitumor composition of ginsenoside. However, the poor water solubility and significant efflux have limited the widespread clinical use of CK. In this study, preparation of novel CK-loaded d-alpha-tocopheryl polyethylene glycol 1,000 succinate/poly(ethylene glycol)-poly(ε-caprolactone) mixed micelles (CK-M) is discussed to solve the above problems. Particle size, zeta potential, and morphology were characterized using dynamic light scattering and transmission electron microscopy. CK-M are spherical shaped with an average particle size of 53.07±1.31 nm with high drug loading of 11.19%±0.87% and entrapment efficiency of 94.60%±1.45%. Water solubility of CK was improved to 3.78±0.09 mg/mL, which was ~107.35 times higher than free CK. A549 and PC-9 cells were used to evaluate in vitro cytotoxicity and cellular uptake. IC50 values of CK-M in A549 and PC-9 cells (24 h) were 25.43±2.18 and 18.35±1.90 μg/mL, respectively. Enhanced cellular uptake of CK-M was observed in both cells. Moreover, CK-M promoted tumor cell apoptosis, inhibited tumor cell invasion, metastasis, and efflux through regulation of Bax, Bcl-2, matrix metalloproteinase-2, Caspase-3, and P-glycoprotei...Continue Reading

Citations

Nov 20, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Zhenghai ZhangShulong Jiang
Dec 18, 2018·Pharmaceutical Development and Technology·Lijun ZhenJiangnan Yu
Feb 8, 2020·Pharmaceutics·Ji-Hyeon JeonIm-Sook Song
Feb 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng XieHaijun Shen
Jul 31, 2020·Pharmaceutical Development and Technology·Liyan ZhaoYiling Wu
Dec 5, 2019·The American Journal of Chinese Medicine·Han XiaoJunling Yang
Jul 27, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·En Hyung Kim, Wonnam Kim
Feb 25, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Heeok HongSeong Gu Hwang
Jan 24, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guobin XuLijun Chen
Nov 19, 2021·Chemical Biology & Drug Design·Li ZhouFan Yang

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Methods Mentioned

BETA
dynamic light scattering
flow cytometry
electrophoresis

Software Mentioned

Graphpad Prism
GraphPad
SPSS13

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