Targeted disruption of Drosophila Roc1b reveals functional differences in the Roc subunit of Cullin-dependent E3 ubiquitin ligases

Molecular Biology of the Cell
Timothy D DonaldsonRobert J Duronio

Abstract

Cullin-dependent ubiquitin ligases regulate a variety of cellular and developmental processes by recruiting specific proteins for ubiquitin-mediated degradation. Cullin proteins form a scaffold for two functional modules: a catalytic module comprised of a small RING domain protein Roc1/Rbx1 and a ubiquitin-conjugating enzyme (E2), and a substrate recruitment module containing one or more proteins that bind to and bring the substrate in proximity to the catalytic module. Here, we present evidence that the three Drosophila Roc proteins are not functionally equivalent. Mutation of Roc1a causes lethality that cannot be rescued by expression of Roc1b or Roc2 by using the Roc1a promoter. Roc1a mutant cells hyperaccumulate Cubitus interruptus, a transcription factor that mediates Hedgehog signaling. This phenotype is not rescued by expression of Roc2 and only partially by expression of Roc1b. Targeted disruption of Roc1b causes male sterility that is partially rescued by expression of Roc1a by using the Roc1b promoter, but not by similar expression of Roc2. These data indicate that Roc proteins play nonredundant roles during development. Coimmunoprecipitation followed by Western or mass spectrometric analysis indicate that the three R...Continue Reading

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Citations

Mar 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mingjia TanYi Sun
May 24, 2008·BMC Genomics·Yongsheng BaiEsther Betrán
Jan 10, 2013·PLoS Pathogens·David J StanleyJohn D Gross
Sep 7, 2011·The Journal of Cell Biology·Jennifer L RohnBuzz Baum
Mar 31, 2007·Trends in Genetics : TIG·Louise V O'KeefeRobert I Richards
Sep 27, 2018·BMC Genomics·Viktor VedelekRita Sinka
Jan 1, 2021·The Journal of Cell Biology·Pedro BarbosaHiroyuki Ohkura
Aug 8, 2021·Developmental Biology·Pourya SarvariDidier Y R Stainier

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