Targeted disruption of Slc2a8 (GLUT8) reduces motility and mitochondrial potential of spermatozoa.

Molecular Membrane Biology
Verena GawlikAnnette Schürmann

Abstract

GLUT8 is a class 3 sugar transport facilitator which is predominantly expressed in testis and also detected in brain, heart, skeletal muscle, adipose tissue, adrenal gland, and liver. Since its physiological function in these tissues is unknown, we generated a Slc2a8 null mouse and characterized its phenotype. Slc2a8 knockout mice appeared healthy and exhibited normal growth, body weight development and glycemic control, indicating that GLUT8 does not play a significant role for maintenance of whole body glucose homeostasis. However, analysis of the offspring distribution of heterozygous mating indicated a lower number of Slc2a8 knockout offspring (30.5:47.3:22.1%, Slc2a8(+/+), Slc2a8(+/-), and Slc2a8(-/-) mice, respectively) resulting in a deviation (p=0.0024) from the expected Mendelian distribution. This difference was associated with lower ATP levels, a reduced mitochondrial membrane potential and a significant reduction of sperm motility of the Slc2a8 knockout in comparison to wild-type spermatozoa. In contrast, number and survival rate of spermatozoa were not altered. These data indicate that GLUT8 plays an important role in the energy metabolism of sperm cells.

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Citations

May 8, 2008·Behavior Genetics·S SchmidtA Schürmann
Jun 1, 2012·Biology of Reproduction·Katie L AdastraKelle H Moley
Aug 23, 2012·PloS One·Nadja MannowetzGunther Wennemuth
Oct 25, 2008·The Journal of Endocrinology·S SchmidtA Schürmann
Dec 25, 2009·Reproductive Biomedicine Online·Ahmet Cem IyibozkurtErkut Attar
Oct 9, 2009·Trends in Endocrinology and Metabolism : TEM·Scott H Purcell, Kelle H Moley
Dec 17, 2014·Molecular Ecology Resources·Michael James StewartJairo Rivera-Posada
Nov 2, 2012·Biology of Reproduction·Theodore ChauvinKenneth P Roberts
Jun 3, 2016·Scientific Reports·Naoki TakedaKen-Ichi Yamamura
Apr 18, 2018·The Journal of Endocrinology·K M JeckelR V Anthony
Jun 28, 2020·Pflügers Archiv : European journal of physiology·Alexandra Chadt, Hadi Al-Hasani
Jan 30, 2009·American Journal of Physiology. Endocrinology and Metabolism·Stefan SchmidtAnnette Schürmann
Jun 7, 2015·The Journal of Biological Chemistry·Maximiliano TourmenteEduardo R S Roldan
Jun 13, 2018·The FEBS Journal·Pierre-Benoit AnceyEtienne Meylan
Oct 21, 2020·Molecular and Cellular Biology·Caroline M AlexanderHeidi Dvinge
Jun 22, 2021·Biochemical and Biophysical Research Communications·Lorena MardonesMarcelo Villagrán

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Methods Mentioned

BETA
PCR
flow cytometry
Assay
electron microscopy

Software Mentioned

CASA

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