PMID: 9657333Jul 10, 1998Paper

Targeted disruption of the bradyzoite-specific gene BAG1 does not prevent tissue cyst formation in Toxoplasma gondii

Molecular and Biochemical Parasitology
W BohneD S Roos

Abstract

Expression of the 30 kDa small heat shock protein BAG1 is restricted to the latent bradyzoite 'tissue cyst' form of Toxoplasma gondii, first appearing approximately 2-3 days after the initiation of bradyzoite differentiation. Although developmental expression of small heat shock proteins has been described for many species, their precise function is unclear. In order to examine the function of BAG1 in T. gondii bradyzoites and its role during parasite differentiation, we have used homologous recombination to produce a knock-out mutant in the cyst-forming strain P(LK), a clonal derivative of ME49. Under tissue culture conditions that stimulate bradyzoite differentiation (alkaline pH), the mutant was found to express several bradyzoite-specific markers with the same kinetics and frequency as the parental strain. Neither enhanced nor decreased susceptibility to stress was observed for the BAG1-deficient mutant. In vivo studies revealed that tachyzoites of the bag1 knock-out mutant were fully able to establish a chronic infection in C57BL/6 mice, producing brain cysts of a size, morphology and frequency indistinguishable from cysts formed by the parental control strain. Brain cysts of the bag1 knock-out mutant contained viable para...Continue Reading

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Citations

Sep 27, 2003·Molecular and Biochemical Parasitology·Jay R RadkeMichael W White
Mar 1, 1997·Molecular and Biochemical Parasitology·W BohneU Gross
May 2, 2002·Trends in Parasitology·Russell E LyonsCraig W Roberts
Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joshua B RadkeMichael W White
Mar 12, 2009·PloS One·Elizabeth A GaskellGlenn A McConkey
Apr 14, 2000·Frontiers in Bioscience : a Journal and Virtual Library·L M Weiss, K Kim
Oct 22, 2008·International Journal for Parasitology·Angelika WagnerUrsula Wiedermann
Jul 24, 2008·Cellular Microbiology·Nathalie VonlaufenWilliam J Sullivan
Aug 25, 2009·Biochimica Et Biophysica Acta·Natalia de MiguelMartin Haslbeck
Jun 6, 2015·Cell Stress & Chaperones·Deyanira Pérez-Morales, Bertha Espinoza
Nov 19, 2011·FEMS Microbiology Reviews·William J Sullivan, Victoria Jeffers
Nov 21, 2007·International Journal for Parasitology·Yasuhiro TakashimaKatsuya Kitoh
Jul 18, 2008·Molecular BioSystems·Marialice da Fonseca Ferreira da SilvaCarsten G K Lüder
Dec 14, 2006·The Journal of Biological Chemistry·Xuchu QueSharon L Reed
Apr 1, 2018·Cellular and Molecular Life Sciences : CMLS·Victoria JeffersWilliam J Sullivan
Dec 19, 2020·ACS Medicinal Chemistry Letters·Muhammad M KhalifaJennifer E Golden

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