Targeted disruption of the GAS41 gene encoding a putative transcription factor indicates that GAS41 is essential for cell viability.

The Journal of Biological Chemistry
Katrin ZimmermannL Phi-van

Abstract

The glioma-amplified sequence (GAS) 41 protein has been proposed to be a transcription factor. To investigate its functional role in vivo, we attempted to knock out the GAS41 gene by targeted disruption in the chicken pre-lymphoid cell line DT40. Heterozygous GAS41+/- cell lines generated by the first round of homologous recombination express approximately half the normal level of GAS41 mRNA. However, a homozygous GAS41-/- cell line with both GAS41 alleles disrupted was not obtained following the second round of transfection, indicating that the GAS41 gene is essential for cell viability. Indeed, homozygous GAS41-/- cell lines with two disrupted GAS41 alleles can be generated following substitution of the endogenous gene by stable integration of GAS41 cDNA controlled by a tetracycline-regulated CMV promoter. Inactivation of this promoter by tetracycline withdrawal results in rapid depletion of GAS41, causing a significant decrease in RNA synthesis and subsequently cell death. Thus, our results indicate that GAS41 is required for RNA transcription.

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S I DworetzkyG S Stein
Feb 25, 1987·Nucleic Acids Research·H J RahmsdorfP Herrlich
Dec 1, 1994·Genes & Development·N L HenryR D Kornberg
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraP C Nowell
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M J MortillaroR Berezney
Jul 11, 1998·The Journal of Biological Chemistry·L Phi-vanW H Strätling
Jul 22, 1998·Nucleic Acids Research·O NaylerS Stamm
Jul 27, 2000·The Journal of Biological Chemistry·J HarborthM Osborn

❮ Previous
Next ❯

Citations

Apr 30, 2013·Cancer Letters·Geun-Hyoung HaEun-Kyoung Yim Breuer
May 4, 2005·Nature Biotechnology·Yun-Han LeeJong-Gu Park
Feb 1, 2003·Nature Reviews. Genetics·William R A BrownStuart A Wilson
Sep 10, 2004·Nucleic Acids Research·Daniel BraasKarl-Heinz Klempnauer
May 18, 2006·Molecular and Cellular Biology·Jeong Hyeon Park, Robert G Roeder
Feb 18, 2004·Molecular and Cellular Biology·Yannick DoyonJacques Côté
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Julia M SchulzeMichael S Kobor
Jul 14, 2010·BMC Molecular Biology·Sabrina HeiselEckart Meese
Feb 16, 2010·Biochimica Et Biophysica Acta·Katrin Hübner, Loc Phi-van
May 11, 2013·The International Journal of Biochemistry & Cell Biology·Xiaofeng DingJian Zhang
Jul 17, 2003·The Journal of Biological Chemistry·Fiona A MyersColyn Crane-Robinson
Jul 20, 2021·Journal of Medicinal Chemistry·Xinyu R MaHong Wen
Aug 4, 2018·ACS Chemical Biology·Hyo Je ChoTomasz Cierpicki

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.