Targeted disruption of the mouse Csrp2 gene encoding the cysteine- and glycine-rich LIM domain protein CRP2 result in subtle alteration of cardiac ultrastructure.

BMC Developmental Biology
Julia F SagaveRalf Weiskirchen

Abstract

The cysteine and glycine rich protein 2 (CRP2) encoded by the Csrp2 gene is a LIM domain protein expressed in the vascular system, particularly in smooth muscle cells. It exhibits a bimodal subcellular distribution, accumulating at actin-based filaments in the cytosol and in the nucleus. In order to analyze the function of CRP2 in vivo, we disrupted the Csrp2 gene in mice and analysed the resulting phenotype. A approximately 17.3 kbp fragment of the murine Csrp2 gene containing exon 3 through 6 was isolated. Using this construct we confirmed the recently determined chromosomal localization (Chromosome 10, best fit location between markers D10Mit203 proximal and D10Mit150 central). A gene disruption cassette was cloned into exon 4 and a mouse strain lacking functional Csrp2 was generated. Mice lacking CRP2 are viable and fertile and have no obvious deficits in reproduction and survival. However, detailed histological and electron microscopic studies reveal that CRP2-deficient mice have subtle alterations in their cardiac ultrastructure. In these mice, the cardiomyocytes display a slight increase in their thickness, indicating moderate hypertrophy at the cellular level. Although the expression of several intercalated disc-associa...Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·R WeiskirchenM C Beckerle
Nov 1, 1993·The Journal of General Virology·A AlcamíE Viñuela
Aug 1, 1997·Molecular and Cellular Biology·Y KongS F Konieczny
Oct 6, 1997·The Journal of Cell Biology·P PomièsM C Beckerle
Jan 24, 1998·Genome Research·L C McCarthyP N Goodfellow
Mar 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J R HendersonM C Beckerle
Aug 5, 2000·Biochemical and Biophysical Research Communications·R Weiskirchen, A M Gressner
May 16, 2001·The Journal of Cell Biology·E EhlerJ C Perriard
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·James R HendersonMary C Beckerle
Jan 18, 2003·Developmental Cell·David F ChangRobert J Schwartz
Jan 23, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ralf Weiskirchen, Kalle Günther
Mar 19, 2003·Circulation·Christian GeierKarl Josef Osterziel
Jan 27, 2004·FEBS Letters·Markus Grubinger, Mario Gimona
Jul 1, 2004·Journal of Cell Science·Preeti AhujaElisabeth Ehler
Sep 20, 2005·Journal of Hepatology·Svenja NeyzenRalf Weiskirchen
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·David F ChangRobert J Schwartz
Aug 22, 2007·Briefings in Functional Genomics & Proteomics·Michael HagnMartin Hrabè de Angelis

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Citations

Feb 18, 2010·International Journal of Cancer. Journal International Du Cancer·Christine WasylykBohdan Wasylyk
Jul 2, 2011·Biochemical and Biophysical Research Communications·Takanori KiharaJun Miyake
Aug 17, 2017·PloS One·Takanori KiharaJun Miyake
Feb 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julia StraubingerRobert Lukowski
Mar 9, 2021·Frontiers in Cardiovascular Medicine·Meihua SheQun Zeng

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Datasets Mentioned

BETA
AY533303

Methods Mentioned

BETA
GTPase
PCR
transfection
genotyping
biopsy
PCRs
electrophoresis
biopsies
electron microscopy
light microscopy

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