Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli

Developmental Dynamics : an Official Publication of the American Association of Anatomists
K I PinsonD L Gumucio

Abstract

The small intestine is functionally dependent on the presence of the brush border, a tightly packed array of microvilli that forms the amplified apical surface of absorptive cells. In the core of each microvillus, actin filaments are bundled by two proteins, villin and fimbrin. Previous in vitro studies using antisense approaches indicated that villin plays an important role in the morphogenesis of microvilli. To examine the in vivo consequences of villin deficiency, we disrupted the mouse villin gene by targeted recombination in mouse embryonic stem cells. A beta-galactosidase cDNA was also introduced into the villin locus by the targeting event. Homozygous villin-deficient mice are viable, fertile, and display no gross abnormalities. Intact microvilli are present in the small intestine, colon, kidney proximal tubules, and liver bile canaliculi. Although subtle ultrastructural abnormalities can be detected in the actin cores of small intestinal microvilli, localization of sucrase isomaltase, brush border myosin I, and zonula occludens I to the microvillar surface of the small intestine is normal. Thus, in vivo, villin plays a minor or redundant role in the generation of microvilli in multiple absorptive tissues.

References

Jun 1, 1978·The Journal of Cell Biology·P Maupin-Szamier, T D Pollard
Jan 1, 1992·Current Topics in Developmental Biology·M B Heintzelman, M S Mooseker
Mar 1, 1992·Analytical Biochemistry·R Ramírez-SolisA Bradley
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E PringaultD Louvard
Feb 1, 1989·The Journal of Cell Biology·L M Coluccio, A Bretscher
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A Bretscher
Mar 1, 1980·The Journal of Cell Biology·S W Craig, C L Lancashire
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J R Glenney, K Weber
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·A Bretscher
Jun 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M M ChafelP Matsudaira
Dec 1, 1994·The Journal of Cell Biology·M ArpinD Louvard
Jan 1, 1993·Methods in Enzymology·C Bonnerot, J F Nicolas
Apr 1, 1994·Molecular and Cellular Biology·C S LinP Matsudaira
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A NagyJ C Roder
Jan 1, 1993·Molecular and Cellular Biology·G KellerM V Wiles
Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Kozak

❮ Previous
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Citations

Oct 11, 2003·Lancet·Peter L M Jansen, Ekkehard Sturm
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·T MijalskiJ Beckers
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Richie KhannaAthar H Chishti
Apr 25, 2002·Molecular and Cellular Biology·Hugh D CampbellKlaus I Matthaei
May 24, 2003·The Journal of Biological Chemistry·David A PotterIra M Herman
Feb 19, 2000·Current Opinion in Cell Biology·J R Bartles
Aug 26, 2003·Molecular Biology of the Cell·Rafika AthmanSylvie Robine
Jul 27, 2004·Nature Medicine·Paul GregorevicJeffrey S Chamberlain
Sep 3, 2004·Molecular Biology of the Cell·Alok TomarSeema Khurana
Mar 27, 2009·Molecular Biology of the Cell·Eva-Maria S Grimm-GünterFrancisco Rivero
Apr 8, 2010·Molecular Pharmacology·Zhanquan ShiDaniel W Nebert
Feb 19, 2002·The European Journal of Neuroscience·Samantha J RavenallArmen N Akopian
Jan 28, 2004·The Journal of Cell Biology·Andrew C Zelhof, Robert W Hardy
Feb 16, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nadine H SoplopSunita G Kramer
Feb 19, 2017·Cold Spring Harbor Perspectives in Biology·Leon J KlunderSven C D van IJzendoorn
Nov 25, 2011·Molecular Biology of the Cell·Céline RevenuSylvie Robine
Jul 7, 2016·Development·Katherine D WaltonDeborah L Gumucio
Feb 4, 2016·Nature Reviews. Gastroenterology & Hepatology·Delphine DelacourDaniel Louvard
Jan 17, 2008·The Journal of Biological Chemistry·Yaohong WangSeema Khurana
Dec 22, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mi-Ran KiKyu-Shik Jeong
May 20, 2020·Journal of Medicinal Food·Kayhan BaşakSedat Aydın
Jun 11, 2020·Molecular Biology of the Cell·Sudeep P GeorgeSeema Khurana
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Oct 28, 2004·Biochemical Society Transactions·S K ArcherH D Campbell
Jul 4, 2007·The Journal of Biological Chemistry·Sudeep P GeorgeSeema Khurana
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Danielle R LittleJichao Chen
Jun 3, 2020·Scientific Reports·Amin EsmaeilniakooshkghaziSeema Khurana
Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rafika AthmanSylvie Robine
Feb 5, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Karen M DownsAllen C Enders
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Evan M BraunsteinDeborah L Gumucio
Oct 10, 2020·Frontiers in Molecular Biosciences·Réka PintérBeáta Bugyi
Mar 2, 2006·Journal of Cell Science·Karin SchlichtingChristian Dahmann
Feb 27, 1999·Current Opinion in Cell Biology·J D Sutherland, W Witke
Feb 19, 2000·Current Opinion in Cell Biology·J A Cooper, D A Schafer

❮ Previous
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