Targeted DNA Methylation Profiling of Human Cardiac Tissue Reveals Novel Epigenetic Traits and Gene Deregulation Across Different Heart Failure Patient Subtypes

Circulation. Heart Failure
Nadezhda GlezevaChris J Watson

Abstract

Limited knowledge exists of the extent of epigenetic alterations, such as DNA methylation, in heart failure (HF). We conducted targeted DNA methylation sequencing to identify DNA methylation alterations in coding and noncoding RNA (ncRNA) across different etiological subtypes of HF. A targeted bisulfite sequence capture sequencing platform was applied to DNA extracted from cardiac interventricular septal tissue of 30 male HF patients encompassing causes including hypertrophic obstructive cardiomyopathy, ischemic cardiomyopathy, dilated cardiomyopathy, and 9 control patients with nonfailing hearts. We detected 62 678 differentially methylated regions in the studied HF cohort. By comparing each HF subgroup to the nonfailing control group, we identified 195 unique differentially methylated regions: 5 in hypertrophic obstructive cardiomyopathy, 151 in dilated cardiomyopathy, and 55 in ischemic cardiomyopathy. These translated to 4 genes/1 ncRNA in hypertrophic obstructive cardiomyopathy, 131 genes/17 ncRNA in dilated cardiomyopathy, and 51 genes/5 ncRNA in ischemic cardiomyopathy. Subsequent gene/ncRNA expression analysis was assessed using quantitative reverse transcription polymerase chain reaction and revealed 6 genes: 4 hyperme...Continue Reading

References

Dec 6, 2005·Human Mutation·Stella Marie Reamon-Buettner, Juergen Borlak
Dec 19, 2012·Gene·Terry S EltonNarasimham L Parinandi
Apr 16, 2013·Journal of Cardiac Failure·Yevgeniya E KoshmanAllen M Samarel
Sep 18, 2014·Circulation. Heart Failure·Thomas G Di Salvo, Saptarsi M Haldar
Nov 10, 2015·Journal of Molecular and Cellular Cardiology·Yevgeniya E KoshmanAllen M Samarel
Feb 26, 2016·Cardiovascular Research·S M J WeltenA Y Nossent
Apr 14, 2016·European Journal of Heart Failure·Francine Z MarquesDavid M Kaye
Apr 25, 2018·Comprehensive Physiology·Adam Russell-HallinanJohn A Baugh

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Citations

Jan 24, 2020·ESC Heart Failure·Takatomo WatanabeGenzou Takemura
Aug 5, 2020·Cardiovascular Drugs and Therapy·Adam Russell-HallinanKarla M O'Neill
Oct 28, 2019·Frontiers in Genetics·Carolina Soler-BotijaAntoni Bayés-Genís
Dec 3, 2020·Journal of Cardiovascular Pharmacology and Therapeutics·Adam Russell-HallinanJohn A Baugh
Jan 8, 2020·JACC. Basic to Translational Science·Chia-Feng Liu, W H Wilson Tang
Jan 23, 2021·Genomics·Robin M W Colpaert, Martina Calore
Jan 28, 2021·Trends in Molecular Medicine·Huiyan LuoRui-Hua Xu
Dec 3, 2020·International Journal of Molecular Sciences·Syeda Shegufta AmeerRalph Knöll
Nov 19, 2020·International Journal of Molecular Sciences·Rui Song, Lubo Zhang
Jun 3, 2021·International Journal of Environmental Research and Public Health·Sek Ying ChairWai Tong Chien
Aug 28, 2021·International Journal of Molecular Sciences·Christina Pagiatakis, Vittoria Di Mauro

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