Abstract
Advanced stage neuroblastoma is an aggressive disease with limited treatment options for patients with drug-resistant tumors. Targeted delivery of chemotherapy for pediatric cancers offers promise to improve treatment efficacy and reduce toxicity associated with systemic chemotherapy. The EnGeneIC Dream Vector (EDVTM) is a nanocell, which can package chemotherapeutic drugs and target tumors via attachment of bispecific proteins to the surface of the nanocell. Phase I trials in adults with refractory tumors have shown an acceptable safety profile. Herein we investigated the activity of EGFR-targeted and doxorubicin-loaded EDVTM (EGFREDVTMDox) for the treatment of neuroblastoma. Two independent neuroblastoma cell lines with variable expression of EGFR protein [SK-N-BE(2), high; SH-SY-5Y, low] were used. EGFREDVTMDox induced apoptosis in these cells compared to control, doxorubicin, or non-doxorubicin loaded EGFREDVTM In three-dimensional tumor spheroids, imaging and fluorescence life-time microscopy revealed that EGFREDVTMDox had a marked enhancement of doxorubicin penetration compared to doxorubicin alone, and improved penetration compared to non-EGFR-targeted EDVTMDox, with enhanced spheroid penetration leading to increased apo...Continue Reading
References
Apr 1, 2004·Annals of Family Medicine·Kevin C OeffingerLeslie L Robison
May 8, 2007·Cancer Cell·Jennifer A MacDiarmidHimanshu Brahmbhatt
Sep 6, 2007·Cell Cycle·Jennifer A MacDiarmidHimanshu Brahmbhatt
Dec 22, 2007·Biophysical Journal·Michelle A DigmanEnrico Gratton
Oct 6, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Suzanne ShustermanPaul M Sondel
Dec 17, 2010·Nature Reviews. Cancer·Chad V PecotAnil K Sood
Feb 12, 2013·Oncogene·F L ByrneM Kavallaris
Apr 25, 2013·Molecular Pharmaceutics·Cyrille BoyerJoshua McCarroll
Dec 10, 2013·Biomacromolecules·Sharon M SagnellaMaria Kavallaris
May 16, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Sharon M SagnellaMaria Kavallaris
Jan 6, 2015·Oncotarget·Joshua A McCarrollMaria Kavallaris
May 21, 2015·American Society of Clinical Oncology Educational Book·Saro H ArmenianCharles Sklar
Aug 19, 2015·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·James R WhittleMark A Rosenthal
Nov 5, 2015·Methods in Molecular Biology·Frances L ByrneMaria Kavallaris
Dec 15, 2015·PloS One·Benjamin J SolomonAndrew M Scott
Apr 5, 2016·Annals of Neurology·Nancy RatnerNina F Schor
Apr 7, 2016·PloS One·Jennifer A MacDiarmidHimanshu Brahmbhatt
Jul 30, 2016·Acta Pharmaceutica Sinica. B·Dinh HaVenkatareddy Nadithe
Citations
Apr 2, 2019·Thyroid : Official Journal of the American Thyroid Association·Lauren Jin Suk JooStan B Sidhu
May 30, 2019·Pharmaceutics·Alessandro ParodiAndrey A Zamyatnin
Dec 26, 2018·Pharmaceutics·Luigi LerraGerardo F Goya
Dec 11, 2019·Polymers·Lisha LiuJianping Liu
Sep 10, 2020·BMC Biomedical Engineering·Kimberly J Ornell, Jeannine M Coburn
Sep 29, 2019·Seminars in Cancer Biology·Faheem Hyder PottooMohammad Amjad Kamal
Jan 1, 2021·Drug Discovery Today·Yan Shan LooThiagarajan Madheswaran
Jan 25, 2021·Advanced Drug Delivery Reviews·Taskeen Iqbal JanjuaAmirali Popat
Mar 19, 2020·Cancer Cell·Sharon M SagnellaHimanshu Brahmbhatt
Apr 4, 2021·Cancers·Caitlin UngDavid S Ziegler
Jun 3, 2021·International Journal of Molecular Sciences·Mariam ElshiatyPetros Christopoulos
Jun 10, 2021·Advanced Science·Yamir IslamTouraj Ehtezazi
Aug 29, 2021·Pharmaceutics·David J LundyPatrick C H Hsieh
Jun 15, 2021·Advanced Materials·Hong PanLintao Cai
Nov 27, 2021·Current Protocols·Andrew J GiffordMichelle Haber