Targeted eicosanoids lipidomics of exhaled breath condensate in healthy subjects

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Marek SanakAndrew Szczeklik

Abstract

Exhaled breath condensate collection is a non-invasive method of sampling the respiratory tract that can be repeated several times in a wide range of clinical settings. Quantitation of non-volatile compounds in the condensate requires highly sensitive analytical methods, e.g. mass spectrometry. To validate cross-platform measurements of eicosanoids using high performance liquid chromatography or gas chromatography coupled with mass spectrometry in exhaled breath condensate sampled from 58 healthy individuals. Twenty different eicosanoid compounds, representing major arachidonic acid lipoxygenation and cyclooxygenation pathways were measured using a stable isotope dilution method. We applied a free palmitic acid concentration as a surrogate marker for the condensate dilution factor. Eicosanoids concentrations in the condensates were consistent with their content in other biological fluids. Prostaglandin E(2) was the most abundant mediator, represented by its stable metabolite tetranor-PGEM. Prostaglandin D(2) products were at low concentration, while hydroxyacids derived from lipoxygenation were abundant. 5-HETE was elevated in current tobacco smokers. Leukotriene B(4) has the highest concentration of all 5-LO products. 15-LO an...Continue Reading

References

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Citations

Mar 27, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Monika JanickaJacek Namieśnik
Jul 5, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kamila SyslováDaniela Pelclová
Feb 15, 2011·The Journal of Allergy and Clinical Immunology·Marek SanakAndrew Szczeklik
Jul 26, 2015·Prostaglandins & Other Lipid Mediators·Lucyna MastalerzMarek Sanak
Mar 16, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W SzczeklikA Szczeklik
Jul 5, 2012·Annals of the New York Academy of Sciences·Jason P EiserichCarroll E Cross
May 19, 2012·Allergy·I AgacheJ C Virchow
Jul 12, 2012·Pediatric Pulmonology·Edyta GlowackaGrzegorz Lis
Oct 10, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Lucyna MastalerzMarek Sanak
Mar 15, 2015·Journal of Pharmaceutical and Biomedical Analysis·Kie KasugaNariyasu Mano
Aug 2, 2015·Analytica Chimica Acta·Antonio ChecaJoaquim Jaumot
May 15, 2015·Biochimica Et Biophysica Acta·Anna PolusAldona Dembinska-Kiec
Feb 18, 2014·The Journal of Allergy and Clinical Immunology·Wei-Jie GuanJin-Ping Zheng
Apr 20, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dimitrios Tsikas, Alexander A Zoerner
Mar 25, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paolo MontuschiNadia Mores
Jul 18, 2016·The Journal of Allergy and Clinical Immunology·Xin FengJohn W Steinke
Aug 10, 2017·The Analyst·O BegouG Theodoridis
Jul 26, 2019·Journal of Breath Research·Alexander J SchmidtCristina E Davis
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Sep 18, 2020·Biomarkers in Medicine·Somayeh KazeminasabMaryam Khoubnasabjafari
Apr 14, 2017·Respiratory Research·Charmion Cruickshank-QuinnNichole Reisdorph
Aug 2, 2012·Journal of Occupational and Environmental Medicine·Nathalie Chérot-KornobisAnnie Sobaszek
Jul 27, 2018·Analytical and Bioanalytical Chemistry·Elaheh RahimpourAbolghasem Jouyban
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Oct 6, 2010·The Journal of Allergy and Clinical Immunology·Christine Sachs-OlsenAndrzej Szczeklik
Jun 4, 2021·International Journal of Chronic Obstructive Pulmonary Disease·Natalia Celejewska-WójcikKrzysztof Sładek
Sep 1, 2017·Analytical Chemistry·Martin Thomas GauggRenato Zenobi

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