Targeted Gene Disruption in Pacific Oyster Based on CRISPR/Cas9 Ribonucleoprotein Complexes

Marine Biotechnology
Hong YuShaojun Du

Abstract

The Pacific oyster (Crassostrea gigas) is a representative bivalve mollusc that is widely cultured in the world. In recent years, it has become an important model species for ecological, evolutionary, and developmental studies because of its ability to survive in extreme environmental conditions as a sessile filter feeder and its classical mosaic pattern of development. Although the complete genome sequence of C. gigas is available and omics data have been rapidly generated for the past few years, the genetic tools for gene functional studies have thus far been limited to RNA interference technology. In this study, we developed a gene editing system for C. gigas based on CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 ribonucleoprotein complexes. Two candidate genes, myostatin (MSTN) and Twist, were selected as targets. After microinjecting CRISPR/Cas9 ribonucleoprotein complexes into fertilized eggs, CRISPR-induced indel mutations were detected in the target genes. The CRISPR/Cas9-induced mutations were predominantly small indel mutations ranging in size from 1 to 24 bp in these two target genes. These results demonstrate that CRISPR/Cas9 can be successfully used as an effective targeted gene editing sy...Continue Reading

References

May 30, 2009·The FEBS Journal·Caroline FabiouxArnaud Huvet
Nov 29, 2014·Science·Jennifer A Doudna, Emmanuelle Charpentier
Dec 23, 2014·Genesis : the Journal of Genetics and Development·Kimberly J Perry, Jonathan Q Henry
Apr 18, 2015·The Journal of Experimental Biology·Arnaud HuvetCaroline Fabioux
May 1, 2015·Genome Biology·Tomomi AidaKohichi Tanaka
Dec 4, 2015·Developmental Biology·Che-Yi Lin, Yi-Hsien Su
Dec 27, 2015·Fish & Shellfish Immunology·Qing WangJianmin Zhao
May 1, 2016·Development·Alexa BurgerChristian Mosimann
Oct 16, 2016·Marine Genomics·Tsuyoshi Momose, Jean-Paul Concordet
Jan 22, 2017·Journal of Insect Physiology·Clauvis Nji Tizi TaningGuy Smagghe
Jun 9, 2017·PLoS Genetics·Guillaume RivierePascal Favrel
Jul 18, 2017·Developmental and Comparative Immunology·Kai SongGuofan Zhang
Sep 16, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chang LiuKun Cheng
May 3, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Chizue HirutaTaisen Iguchi
May 16, 2018·Fungal Genetics and Biology : FG & B·Qiang WangJeffrey J Coleman
Oct 26, 2018·Developmental Biology·Maryam MehravarMehdi Banan

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Citations

May 24, 2019·Marine Biotechnology·Jianfang HuangCaihuan Ke
Apr 16, 2020·Evolution & Development·Zhihui YangShi Wang
Jan 4, 2021·Cellular and Molecular Life Sciences : CMLS·Yu-Fan ChuangGuei-Sheung Liu
May 26, 2020·Fish & Shellfish Immunology·Adrienne N TracyJosé A Fernández Robledo
Mar 11, 2021·International Journal of Biological Macromolecules·Huijuan LiQi Li
Apr 6, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Robert W A PottsRoss D Houston
Apr 6, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Angus Davison, Maurine Neiman

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Methods Mentioned

BETA
gene knockout
targeted genetic mutations
PCR
in
electrophoresis
fluorescence microscopy

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