Targeted gene therapy of nasopharyngeal cancer in vitro and in vivo by enhanced thymidine kinase expression driven by human TERT promoter and CMV enhancer.

Journal of Experimental & Clinical Cancer Research : CR
Cong-Xiang ShenXiao-Fang Guan

Abstract

To explore the therapeutic effects of thymidine kinase (TK) expressed by enhanced vector pGL3-basic- hTERTp-TK-EGFP-CMV driven by human telomerase reverse transcriptase promoter (hTERTp) as well as cytomegalovirus immediate early promoter enhancer (CMV). Enhanced TK-EGFP expression was confirmed by fluorescent microscopy, real time PCR and telomerase activity. Its effects were examined by survival of tumor cells NPC 5-8F and MCF-7, index of xenograft implanted in nude mice and histology. Compared with non-enhanced vector pGL3-basic-TK-hTERTp-EGFP, TK expressed by the enhanced vector significantly decreased NPC 5-8F and MCF-7 cell survival rates after ganciclovir (GCV) treatment (p < 0.001) and tumor progress in nude mice with NPC xenograft and treated with GCV, without obvious toxicity to mouse liver and kidney. The enhanced TK expression vector driven by hTERTp with CMV enhancer has brighter clinical potentials in nasopharyngeal carcinoma therapy than the non-enhanced vector.

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Citations

Feb 10, 2012·Journal of Experimental & Clinical Cancer Research : CR·Xiao-Fen LaiHai-Li Wang
Jul 14, 2012·Expert Opinion on Biological Therapy·Donavon C Hiss, Burtram C Fielding
Nov 9, 2011·Journal of the Korean Surgical Society·Sun Choon SongMin Jung Kim

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Datasets Mentioned

BETA
AY575228

Methods Mentioned

BETA
PCR
transfection
light microscopy
xenograft

Software Mentioned

SPSS11

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