Targeted high-throughput sequencing identifies pathogenic mutations in KCNQ4 in two large Chinese families with autosomal dominant hearing loss

PloS One
Hongyang WangQiuju Wang

Abstract

Autosomal dominant non-syndromic hearing loss (ADNSHL) is highly heterogeneous, among them, KCNQ4 is one of the most frequent disease-causing genes. More than twenty KCNQ4 mutations have been reported, but none of them were detected in Chinese mainland families. In this study, we identified a novel KCNQ4 mutation in a five generation Chinese family with 84 members and a known KCNQ4 mutation in a six generation Chinese family with 66 members. Mutation screening of 30 genes for ADNSHL was performed in the probands from thirty large Chinese families with ADNSHL by targeted region capture and high-throughput sequencing. The candidate variants and the co-segregation of the phenotype were verified by polymerase chain reaction (PCR) amplification and Sanger sequencing in all ascertained family members. Then we identified a novel KCNQ4 mutation p.W275R in exon 5 and a known KCNQ4 mutation p.G285S in exon 6 in two large Chinese ADNSHL families segregating with post-lingual high frequency-involved and progressive sensorineural hearing loss. This is the first report of KCNQ4 mutation in Chinese mainland families. KCNQ4, a member of voltage-gated potassium channel family, is likely to be a common gene in Chinese patients with ADNSHL. The r...Continue Reading

References

Apr 15, 2018·Molecular Genetics & Genomic Medicine·Hongyang WangQiuju Wang
Jan 23, 2018·Science China. Life Sciences·Hongyang WangQiuju Wang
Oct 6, 2016·Journal of Cellular Physiology·Rahul MittalXue Zhong Liu
Jun 21, 2018·Frontiers in Molecular Neuroscience·Qing-Qing HaoShi-Ming Yang

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Related Concepts

Tryptophan
Audiometry
Exertion
Exons
Aminoglycosides
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Pathogenic Organism
Hearing Tests
Western Blotting
Genetic Screening Method

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