Targeted Imaging Agent to HSP70 Induced In Vivo.

Molecular Imaging
Pradip GhoshKing C Li

Abstract

Heat shock protein expression can be induced by heat shock making it possible to artificially modulate their levels noninvasively in vivo in a spatially and temporally controlled manner. Here, we report the use of the major heat shock protein 70 (HSP70) as an inducible target by using the small molecule deoxyspergualin (DSG) conjugated to the near-infrared fluorophore (Cy5.5). We demonstrate that heat induction in the form of localized hyperthermia of normal tissue in living mice results in sufficient HSP70 overexpression for detection with DSG-Cy5.5 conjugate. This effect is dependent on total energy delivered and reaches maximum fluorescence signal in 6 to 8 hours post heat induction and declines over a period of up to 24 hours. These results suggest that DSG-Cy5.5 agent accumulates in tissue with elevated HSP70 by heat.

References

Aug 1, 1992·International Journal of Peptide and Protein Research·Z TianR W Roeske
Feb 1, 1991·Journal of Medicinal Chemistry·M L EdwardsA Sjoerdsma
Nov 1, 1988·The Journal of Antibiotics·R NishizawaT Nakamura
Jul 1, 1987·The Journal of Antibiotics·K NemotoH Umezawa
Dec 1, 1981·The Journal of Antibiotics·T TakeuchiH Umezawa
Dec 1, 1981·The Journal of Antibiotics·H UmezawaT Takeuchi
Dec 1, 1981·The Journal of Antibiotics·S KondoH Umezawa
Jan 17, 1996·Biochemical and Biophysical Research Communications·J S SeoW H Lee
Apr 13, 2004·Oncogene·Dick D Mosser, Richard I Morimoto
Mar 3, 2005·Genes & Development·Mikkel RohdeMarja Jäättelä
Jul 26, 2005·Cell Stress & Chaperones·Daniel R Ciocca, Stuart K Calderwood
Sep 7, 2005·European Journal of Immunology·Stuart K CalderwoodJianlin Gong
Sep 20, 2005·British Journal of Pharmacology·Csaba SõtiPéter Ferdinandy
Jan 18, 2006·Journal of Pediatric Surgery·John A SandovalLinda H Malkas
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Alberto J L Macario, Everly Conway de Macario
Jun 15, 2007·FEBS Letters·Marissa V Powers, Paul Workman
Apr 2, 2009·Expert Opinion on Therapeutic Targets·Luke Whitesell, Susan Lindquist
May 15, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Gang NiuXiaoyuan Chen
Jan 11, 2011·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Pradip GhoshDaniel Y Lee
Sep 8, 2011·Methods in Molecular Biology·Anne-Laure RéroleCarmen Garrido
Mar 30, 2013·Functional & Integrative Genomics·Mehdi MoghanibashiParisa Mohamadynejad
Apr 9, 2013·Carcinogenesis·Maureen E Murphy
May 3, 2014·International Journal of Oncology·Xiaoxia WangXu Zhang
Aug 29, 2014·PloS One·Mathias GehrmannGabriele Multhoff

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
NMR
fluorescence microscopy
column chromatography
acylation

Software Mentioned

Living Image

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Y H ItohM Morita
Journal of Medicinal Chemistry
Christopher G EvansJason E Gestwicki
The Journal of Immunology : Official Journal of the American Association of Immunologists
R DresselE Günther
© 2021 Meta ULC. All rights reserved