Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination.

Genes & Development
Frank R NeumannSusan M Gasser

Abstract

Chromatin in the interphase nucleus moves in a constrained random walk. Despite extensive study, the molecular causes of such movement and its impact on DNA-based reactions are unclear. Using high-precision live fluorescence microscopy in budding yeast, we quantified the movement of tagged chromosomal loci to which transcriptional activators or nucleosome remodeling complexes were targeted. We found that local binding of the transcriptional activator VP16, but not of the Gal4 acidic domain, enhances chromatin mobility. The increase in movement did not correlate strictly with RNA polymerase II (PolII) elongation, but could be phenocopied by targeting the INO80 remodeler to the locus. Enhanced chromatin mobility required Ino80's ATPase activity. Consistently, the INO80-dependent remodeling of nucleosomes upon transcriptional activation of the endogenous PHO5 promoter enhanced chromatin movement locally. Finally, increased mobility at a double-strand break was also shown to depend in part on the INO80 complex. This correlated with increased rates of spontaneous gene conversion. We propose that local chromatin remodeling and nucleosome eviction increase large-scale chromatin movements by enhancing the flexibility of the chromatin f...Continue Reading

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J E GermondP Chambon
Nov 1, 1992·Trends in Genetics : TIG·F Winston, M Carlson
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B C LaurentM Carlson
Oct 6, 1988·Nature·I SadowskiM Ptashne
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·J H WilsonJ E Haber
Feb 21, 1998·Current Biology : CB·W F MarshallJ W Sedat
Dec 12, 2001·Science·P HeunS M Gasser
Mar 23, 2002·Current Biology : CB·Jonathan R ChubbWendy A Bickmore
Nov 16, 2002·Science·David J StegerErin K O'Shea
Mar 7, 2003·Molecular Cell·Stefan R KassabovBlaine Bartholomew
Jun 4, 2004·Molecular Cell·Hinrich BoegerRoger D Kornberg
Jun 16, 2004·Current Opinion in Genetics & Development·Andrew Flaus, Tom Owen-Hughes
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kerstin BystrickySusan M Gasser
Jan 20, 2005·Molecular and Cellular Biology·Anne E CarpenterAndrew S Belmont
Aug 6, 2005·Cell Cycle·Haico van Attikum, Susan M Gasser
Sep 30, 2005·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Daniel SageMichael Unser
Apr 25, 2006·Current Biology : CB·Chien-Hui ChuangAndrew S Belmont
May 1, 2007·Current Opinion in Cell Biology·Angela Taddei
Jul 17, 2007·The Journal of Biological Chemistry·Slobodan BarbaricPhilipp Korber
Nov 28, 2007·The Journal of Cell Biology·Jennifer M BuppSue L Jaspersen
Jan 24, 2008·Molecular and Cellular Biology·Sushma Shivaswamy, Vishwanath R Iyer
Apr 15, 2008·Current Biology : CB·Kenji ShimadaSusan M Gasser
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Randall S Gieni, Michael J Hendzel

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Citations

Aug 8, 2013·DNA Repair·Anastas Gospodinov, Zdenko Herceg
Aug 21, 2013·Mutation Research·Irene ChioloSylvain V Costes
Jul 23, 2013·Trends in Cell Biology·Judith Miné-Hattab, Rodney Rothstein
Jul 3, 2013·Nature Communications·Gwendolyn BennettCraig L Peterson
Dec 19, 2012·Nature Reviews. Genetics·Manolis Papamichos-Chronakis, Craig L Peterson
Jul 31, 2012·Nucleic Acids Research·Davide MazzaJames G McNally
Sep 12, 2012·Genetics·Angela Taddei, Susan M Gasser
Feb 26, 2014·The Journal of Cell Biology·Tsuyoshi Morita, Ken'ichiro Hayashi
Dec 18, 2013·Genome Biology·Ishita S MehtaBasuthkar J Rao
Aug 5, 2014·Trends in Cell Biology·Christian B Gerhold, Susan M Gasser
Feb 2, 2016·Nature Cell Biology·Jonathan StreckerDaniel Durocher
Aug 17, 2014·Journal of Molecular Biology·Ling PanLi-Huei Tsai
Jan 21, 2016·Biophysical Journal·Thomas J LampoAndrew J Spakowitz
Dec 3, 2014·Journal of Molecular Biology·Charlène Lemaître, Evi Soutoglou
Dec 3, 2014·Journal of Molecular Biology·Christian-Benedikt GerholdSusan M Gasser
Feb 2, 2013·Mechanisms of Ageing and Development·Alan M Weiner, Lucas T Gray
Jul 11, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Zhijun Duan, Carl Anthony Blau
Sep 11, 2013·EMBO Reports·Vincent DionSusan M Gasser
Nov 19, 2014·Molecular Cancer·Olga V IarovaiaYegor S Vassetzky
Apr 3, 2015·The Plant Journal : for Cell and Molecular Biology·Chi ZhangAiwu Dong
Jan 29, 2013·Current Opinion in Genetics & Development·Andrew SeeberSusan M Gasser
Aug 27, 2013·Nucleus·Hua WongChristophe Zimmer
Sep 12, 2013·Nucleus·Babett SteglichKarl Ekwall
Nov 26, 2013·Molecular Cell·Jolien Suzanne VerdaasdonkKerry Bloom
Mar 19, 2013·Cell·Vincent Dion, Susan M Gasser
Mar 26, 2013·Molecular Cell·Jörg RenkawitzStefan Jentsch
May 9, 2016·Trends in Biochemical Sciences·Jason C Bell, Stephen C Kowalczykowski
Jun 9, 2016·DNA Repair·Yoshiko KubotaSaburo Horiuchi
Jul 5, 2016·Frontiers in Genetics·Shane M Harding, Roger A Greenberg
Nov 5, 2016·Current Opinion in Genetics & Development·Andrew Seeber, Susan M Gasser
Mar 7, 2017·Nature Structural & Molecular Biology·François AymardGaëlle Legube
Apr 8, 2014·Methods : a Companion to Methods in Enzymology·Yusuke Miyanari
Aug 11, 2017·Molecular Biology of the Cell·Judith Miné-HattabXavier Darzacq

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