Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis

Molecular Plant
Gao-Jun LiuYuxian Zhu

Abstract

The membrane lipids from fast-elongating wild-type cotton (Gossypium hirsutum) fibers at 10 days post-anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. Fiber cells contained significantly higher amounts of phosphatidylinositol (PI) than both ovule samples with PI 34:3 being the most predominant species. The genes encoding fatty acid desaturases (Δ(15)GhFAD), PI synthase (PIS) and PI kinase (PIK) were expressed in a fiber-preferential manner. Further analysis of phosphatidylinositol monophosphate (PIP) indicated that elongating fibers contained four- to five-fold higher amounts of PIP 34:3 than the ovules. Exogenously applied linolenic acid (C18:3), soybean L-α-PI, and PIPs containing PIP 34:3 promoted significant fiber growth, whereas a liver PI lacking the C18:3 moiety, linoleic acid, and PIP 36:2 were completely ineffective. The growth inhibitory effects of carbenoxolone, 5-hydroxytryptamine, and wortmannin were reverted by C18:3, PI, or PIP, respectively, suggesting that PIP signaling is essential for fiber cell growth. Furthermore, cotton plants expressing virus-induced gene-silencing constructs that specifi...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J B OhlroggeP K Stumpf
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·B BrüggerW D Lehmann
Jul 20, 1999·European Journal of Biochemistry·S CollinJ C Kader
May 4, 2000·Progress in Lipid Research·L Dircks, H S Sul
Feb 22, 2005·Journal of Lipid Research·Eoin FahyEdward A Dennis
Aug 22, 2007·Current Opinion in Plant Biology·Hongwei XueGang Li
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Jun 11, 2009·Plant Signaling & Behavior·Ingo Heilmann
Oct 15, 2010·Current Opinion in Plant Biology·Yong-Mei Qin, Yu-Xian Zhu
Dec 15, 2010·Cell·Markus R Wenk
Apr 8, 2011·Annual Review of Biochemistry·Richard Harkewicz, Edward A Dennis
Jul 22, 2011·Current Opinion in Plant Biology·Teun Munnik, Erik Nielsen
Feb 18, 2012·The Plant Cell·Patrick J HornKent D Chapman
May 1, 2012·Progress in Lipid Research·Dong-Soon Im
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Sep 17, 2013·Advances in Nutrition·Alfred H MerrillEoin Fahy
Feb 14, 2014·Plant Biotechnology Journal·Zhouhang GuXueping Zhou

❮ Previous
Next ❯

Citations

Mar 5, 2016·Frontiers in Plant Science·Ibrokhim Y AbdurakhmonovAlan E Pepper
Nov 1, 2016·Molecular Genetics and Genomics : MGG·Xiaoguang ShangWangzhen Guo
Apr 21, 2017·Frontiers in Plant Science·Yuzhou ZhangGuanghui Xiao
Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liping ZhuHongbin Li
Jan 26, 2020·Trends in Plant Science·Zuoren YangFuguang Li
May 17, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Milada VítováSamuel Furse

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.