Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma

Scientific Reports
Lucas A TomkoSuzanne M Ponik

Abstract

Increasing evidence demonstrates an important role for the extracellular matrix (ECM) in breast cancer progression. Collagen type I, a core constituent of the fibrous ECM, undergoes a significant set of changes that accompany tumor progression, termed Tumor Associated Collagen Signatures (TACS). Late stages of this progression are characterized by the presence of bundled, straight collagen (TACS-2) that become oriented perpendicular to the tumor-stromal boundary (TACS-3). Importantly, the presence of TACS-3 collagen is an independent predictor of poor patient outcome. At present, it remains unclear whether reorganization of the collagen matrix is the consequence of mechanical or compositional tissue remodeling. Here, we identify compositional changes in ECM correlating to collagen fiber reorganization from nineteen normal and invasive ductal carcinoma (IDC) patient biopsies using matrisome-targeted proteomics. Twenty-seven ECM proteins were significantly altered in IDC samples compared to normal tissue. Further, a set of nineteen matrisome proteins positively correlate and five proteins inversely correlate with IDC tissues containing straightened collagen fibers. Tenascin-C and thrombospondin-2 significantly co-localized with a...Continue Reading

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Citations

Aug 30, 2019·Essays in Biochemistry·Isra N Taha, Alexandra Naba
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Sep 18, 2021·Journal of Proteome Research·Marina Trombetta-LimaGiuseppe Palmisano

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Methods Mentioned

BETA
biopsy
biopsies
Protein Assay
Fluorescence Imaging

Software Mentioned

[UNK]
Proteome
ct
- Plotter
MetaboAnalyst
FIRE
ctFIRE
FIJI
KM
Mascot

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