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Targeted mutagenesis using CRISPR/Cas system in medaka

Biology Open

Apr 15, 2014

Satoshi Ansai, Masato Kinoshita

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful targeted mutagenesis using RGENs in...read more

Mentioned in this Paper

Embryo
Genome
Endonuclease
Promoter
Genomics
Nuclease
Protein Domain
PARK7 wt Allele
Clustered Regularly Interspaced Short Palindromic Repeats
Mutagenesis Procedure
23
177
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Targeted mutagenesis using CRISPR/Cas system in medaka

Biology Open

Apr 15, 2014

Satoshi Ansai, Masato Kinoshita

PMID: 24728957

DOI: 10.1242/bio.20148177

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful targeted mutagenesis using RGENs in...read more

Mentioned in this Paper

Embryo
Genome
Endonuclease
Promoter
Genomics
Nuclease
Protein Domain
PARK7 wt Allele
Clustered Regularly Interspaced Short Palindromic Repeats
Mutagenesis Procedure
23
177

Feeds With Similar Papers

Embryonic Development: Enhancer/Promoter(Keystone)

During development and differentiation of an organism, accurate gene regulation is central for cells to maintain and balance their differentiation processes. Transcriptional interactions between cis-acting DNA elements such as promoters and enhancers are the basis for precise and balanced transcriptional regulation.This feed follows research on enhancer promoter communication in embryonic development.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

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  • References
  • Citations37
  • finger pointing at paper

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    We're still populating references for this paper, please check back later.
  • References
  • Citations37
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