PMID: 9546185Apr 18, 1998Paper

Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases

Applied and Environmental Microbiology
John S Scott-CraigJ D Walton

Abstract

The filamentous fungus Cochliobolus carbonum produces endo-alpha 1,4-polygalacturonase (endoPG), exo-alpha 1,4-polygalacturonase (exoPG), and pectin methylesterase when grown in culture on pectin. Residual activity in a pgn1 mutant (lacking endoPG) was due to exoPG activity, and the responsible protein has now been purified. After chemical deglycosylation, the molecular mass of the purified protein decreased from greater than 60 to 45 kDa. The gene that encodes exoPG, PGX1, was isolated with PCR primers based on peptide sequences from the protein. The product of PGX1, Pgx1p, has a predicted molecular mass of 48 kDa, 12 potential N-glycosylation sites, and 61% amino acid identity to an exoPG from the saprophytic fungus Aspergillus tubingensis. Strains of C. carbonum mutated in PGX1 were constructed by targeted gene disruption and by gene replacement. Growth of pgx1 mutant strains on pectin was reduced by ca. 20%, and they were still pathogenic on maize. A double pgn1/pgx1 mutant strain was constructed by crossing. The double mutant grew as well as the pgx1 single mutant on pectin and was still pathogenic despite having less than 1% of total wild-type PG activity. Double mutants retained a small amount of PG activity with the sam...Continue Reading

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Citations

Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·L M RogersP E Kolattukudy
Apr 25, 2001·Applied and Environmental Microbiology·F I García-MaceiraM I Roncero
Jul 1, 2001·Molecular Plant Pathology·A Idnurm, B J Howlett
Dec 30, 2015·Molecular Plant-microbe Interactions : MPMI·Luca SellaFrancesco Favaron
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Odile Valette-ColletMartine Boccara
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·Dani EshelDov Prusky
Mar 7, 2008·Molecular Plant-microbe Interactions : MPMI·Chih-Hang WuRuey-Fen Liou
Apr 10, 2015·International Journal of Molecular Sciences·Zhangyong Dong, Zhenzhong Wang
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·A ten HaveJ A van Kan
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·H KimJ D Walton
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Eugenia Morán-DiezEnrique Monte
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Arvid GötessonAdrienne R Hardham
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Enid T González, Caitilyn Allen
Jul 27, 2001·Bioscience, Biotechnology, and Biochemistry·J H AhnJ D Walton
Nov 1, 2003·Molecular Plant Pathology·Dov Prusky, Nir Yakoby
Mar 22, 2013·Molecular Biology and Evolution·Patrick C BrunnerBruce A McDonald
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·F I García-MaceiraM I Roncero
Aug 13, 2017·Molecular Plant-microbe Interactions : MPMI·Maria Chiara PaccanaroFrancesco Favaron
Sep 7, 2019·Current Protein & Peptide Science·Verónica PlazaLuis Castillo
Aug 11, 2001·Molecular Plant-microbe Interactions : MPMI·N YakobyD Prusky
Jul 18, 2001·Fungal Genetics and Biology : FG & B·A ten HaveJ A van Kan
Mar 23, 2002·Phytochemistry·Frédéric BrunnerThorsten Nürnberger
Mar 26, 2002·Microbiological Research·Maria Angélica dos Santos Cunha ChellegattiSuraia Said
Oct 22, 2008·Fungal Genetics and Biology : FG & B·Jeffrey A MertensAlejandro P Rooney

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