Targeted Neurotransmitters Profiling Identifies Metabolic Signatures in Rat Brain by LC-MS/MS: Application in Insomnia, Depression and Alzheimer's Disease

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Huarong XuQing Li

Abstract

Epidemiological, cross-sectional, and prospective studies have suggested that insomnia, Alzheimer's disease (AD) and depression are mutually interacting conditions and frequently co-occur. The monoamine and amino acid neurotransmitter systems in central nervous system were involved in the examination of neurobiological processes of this symptom complex. However, few studies have reported systematic and contrastive discussion of different neurotransmitters (NTs) changing in these neurological diseases. Thus, it is necessary to establish a reliable analytical method to monitoring NTs and their metabolite levels in rat brain tissues for elucidating the differences in pathophysiology of these neurological diseases. A rapid, sensitive and reliable LC-MS/MS method was established for simultaneous determination of the NTs and their metabolites, including tryptophan (Trp), tyrosine (Tyr), serotonin (5-HT), 5-hydroxyindolacetic acid (5-HIAA), dopamine (DA), acetylcholine (ACh), norepinephrine (NE), glutamic acid (Glu), and γ-aminobutyric acid (GABA) in rat brain tissues. The mobile phase consisting of methanol and 0.01% formic acid in water was performed on an Inertsil EP C18 column, and the developed method was validated well. Results ...Continue Reading

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Citations

Aug 1, 2020·Endocrinology·Alana M C Brown, Nicole J Gervais
May 20, 2020·Frontiers in Bioengineering and Biotechnology·Kai MiDesi Shang
Oct 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Shaodong DengHaiyi Ye
Jun 15, 2021·Pharmacological Reports : PR·Karolina MaciejewskaPaweł Szymański
Aug 21, 2021·Frontiers in Psychiatry·Lining DuanYousheng Mo

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Methods Mentioned

BETA
deamination
electrophoresis
feature extraction

Software Mentioned

SPSS
Analyst

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