Targeted polymeric micelles for siRNA treatment of experimental cancer by intravenous injection

ACS Nano
R James ChristieKazunori Kataoka

Abstract

Small interfering ribonucleic acid (siRNA) cancer therapies administered by intravenous injection require a delivery system for transport from the bloodstream into the cytoplasm of diseased cells to perform the function of gene silencing. Here we describe nanosized polymeric micelles that deliver siRNA to solid tumors and elicit a therapeutic effect. Stable multifunctional micelle structures on the order of 45 nm in size formed by spontaneous self-assembly of block copolymers with siRNA. Block copolymers used for micelle formation were designed and synthesized to contain three main features: a siRNA binding segment containing thiols, a hydrophilic nonbinding segment, and a cell-surface binding peptide. Specifically, poly(ethylene glycol)-block-poly(L-lysine) (PEG-b-PLL) comprising lysine amines modified with 2-iminothiolane (2IT) and the cyclo-Arg-Gly-Asp (cRGD) peptide on the PEG terminus was used. Modification of PEG-b-PLL with 2IT led to improved control of micelle formation and also increased stability in the blood compartment, while installation of the cRGD peptide improved biological activity. Incorporation of siRNA into stable micelle structures containing the cRGD peptide resulted in increased gene silencing ability, im...Continue Reading

References

Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Nov 1, 1996·Journal of Neuropathology and Experimental Neurology·C L Gladson
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Mar 4, 2000·Advanced Drug Delivery Reviews·R WattiauxM Jadot
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Dec 19, 2003·Nucleic Acids Research·Raphaël LeplaeAriane Toussaint
Nov 3, 2004·Nucleic Acids Research·Raymond M SchiffelersMartin C Woodle
Dec 9, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel J Hicklin, Lee M Ellis
Feb 16, 2006·Drug Discovery Today·Frank Y XiePatrick Y Lu
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Xavier MontetLee Josephson
May 23, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Femke M van de WaterRosalinde Masereeuw
Aug 16, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Ingrid DijkgraafOtto C Boerman
Aug 31, 2006·Cell Cycle·Charles X LiJohannes H Fruehauf
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Sep 25, 2007·Nature Biotechnology·Hak Soo ChoiJohn V Frangioni
Jan 31, 2008·Human Gene Therapy·Adam Judge, Ian MacLachlan
Jan 1, 2009·Molecular Pharmaceutics·Kun Gao, Leaf Huang
Jan 23, 2009·Nature·Daniela Castanotto, John J Rossi
Jan 31, 2009·Nature Reviews. Drug Discovery·Kathryn A WhiteheadDaniel G Anderson
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Mar 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lucie SanceyColl Jean-Luc
Mar 19, 2009·Molecular Pharmaceutics·James J LuJianzhu Chen
Apr 10, 2009·Advanced Drug Delivery Reviews·Kenneth Alan Howard
May 16, 2009·Oligonucleotides·Marjorie RobbinsIan MacLachlan
Sep 10, 2010·Chembiochem : a European Journal of Chemical Biology·Carmen WänglerBjörn Wängler
Mar 5, 2011·Small·Shawn J TanDan Luo
Mar 8, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takahiro NomotoKazunori Kataoka
Jul 16, 2011·Bioconjugate Chemistry·Md Rowshon AlamRudy L Juliano

❮ Previous
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Citations

Dec 18, 2012·Pharmaceutical Research·Mengjie RuiYuhong Xu
Oct 2, 2012·Journal of the American Chemical Society·Ke ZhangChad A Mirkin
Jul 3, 2013·Biological & Pharmaceutical Bulletin·Makoto Oba
Dec 5, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takahiro NomotoKazunori Kataoka
Jun 7, 2014·Annual Review of Biomedical Engineering·John-Michael WillifordHai-Quan Mao
May 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·M HyodoH Harashima
Nov 22, 2014·Nanomedicine·Szu-Ting Chou, A James Mixson
Jul 19, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiyu KeYi Yan Yang
Jul 9, 2013·Nanoscale·Eric BusseronNicolas Giuseppone
Jan 21, 2016·Expert Opinion on Drug Delivery·Wajd Amly, Rafik Karaman
Jul 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Horacio Cabral, Kazunori Kataoka
Feb 3, 2016·Molecular Pharmaceutics·Sonke SvensonScott Eliasof
Dec 15, 2015·Chemical Communications : Chem Comm·Takehiko IshiiKazunori Kataoka
Dec 3, 2015·Advanced Healthcare Materials·Leonard KapsRudolf Zentel
May 17, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kumiko Sakai-KatoToru Kawanishi
Apr 6, 2016·Regenerative Biomaterials·Ling LiYu-Quan Wei
Jul 6, 2013·Journal of Biomedical Materials Research. Part a·Yi-Chun ChenGing-Ho Hsiue
Jul 4, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Kristen L KozielskiJordan J Green
Mar 17, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Haruka NishidaTomoyuki Fujii
Jul 15, 2015·Bioconjugate Chemistry·Dorothy W Pan, Mark E Davis
Mar 8, 2013·International Journal of Pharmaceutics·Jianfeng GuoCaitriona M O'Driscoll
Mar 31, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Edgar Pérez-Herrero, Alberto Fernández-Medarde
Oct 15, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu SakuraiHideyoshi Harashima
May 6, 2014·Frontiers in Pharmacology·Aditi M Jhaveri, Vladimir P Torchilin
Nov 11, 2014·Molecular Pharmaceutics·Luís NovoWim E Hennink
Jun 2, 2016·Expert Opinion on Drug Delivery·Kristen L KozielskiJordan J Green
Apr 27, 2016·Cancer Science·Nobuhiro NishiyamaKazunori Kataoka
Apr 5, 2014·Biomacromolecules·Lutz NuhnRudolf Zentel
Oct 23, 2014·Biomacromolecules·Lutz NuhnRudolf Zentel

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