Targeted production of secondary metabolites by coexpression of non-ribosomal peptide synthetase and prenyltransferase genes in Aspergillus

Applied Microbiology and Biotechnology
Carsten WunschShu-Ming Li

Abstract

Prenylated tryptophan-containing cyclic dipeptides are found in different fungi and serve as precursors for the biosynthesis of diverse biologically active secondary metabolites. They show distinct and usually higher biological and pharmacological activities than the respective non-prenylated dipeptides. Successful production of such compounds were achieved by a new approach based on the coexpression of ftmPS, a non-ribosomal peptide synthetase from Neosartorya fischeri, with three cyclic dipeptide prenyltransferase genes from different biosynthetic gene clusters in Aspergillus nidulans. The genes are expressed under the control of constitutive gpdA promoter and trpC terminator. Expression of ftmPS alone resulted in the formation of the expected cyclic dipeptide brevianamide F with a yield of up to 36.9 mg l(-1). Introducing the reverse C2-prenyltransferase gene cdpC2PT as well as the reverse C3-prenyltransferase gene cdpNPT into a ftmPS mutant yielded reversely C2- and C3-prenylated derivatives, respectively. Coexpression of ftmPS with the reverse C3-prenyltransferase gene cdpC3PT resulted in the formation of N1-regularly, C2-, and C3-reversely prenylated derivatives. The prenyl transfer reactions catalyzed by CdpC2PT, CdpNPT,...Continue Reading

References

Jul 27, 1992·FEBS Letters·M A Marahiel
Apr 15, 1983·Biochemical and Biophysical Research Communications·D J BallanceG Turner
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M YeltonW E Timberlake
Dec 1, 1983·Gene·J TilburnR W Davies
Jul 25, 1998·The Biochemical Journal·T UsuiH Osada
Jan 5, 2001·Bioorganic & Medicinal Chemistry Letters·A van LoevezijnG J Koomen
Feb 20, 2002·Chemical Reviews·Robert E. Minto, Craig A. Townsend
Aug 30, 2003·The Journal of Organic Chemistry·Esmeralda CaballeroJ Carlos Menéndez
Aug 6, 2004·Applied and Environmental Microbiology·Ana M CalvoNancy P Keller
Aug 12, 2004·Nucleic Acids Research·Lei ZhengJean-Louis Reymond
Jun 7, 2006·Chembiochem : a European Journal of Chemical Biology·Shubha MaiyaGeoffrey Turner
May 26, 2007·Chembiochem : a European Journal of Chemical Biology·Wen-Bing YinShu-Ming Li
Sep 3, 2008·Medical Mycology·Jens C FrisvadThomas O Larsen
Apr 18, 2009·Fungal Genetics and Biology : FG & B·Shubha MaiyaGeoffrey Turner
Mar 17, 2010·Biochemistry·Brian D Ames, Christopher T Walsh
May 4, 2010·Fungal Genetics and Biology : FG & B·Axel A Brakhage, Volker Schroeckh
Nov 11, 2010·The Journal of Antibiotics·Shu-Ming Li
May 12, 2011·Nucleic Acids Research·Marc RöttigOliver Kohlbacher
Nov 16, 2011·Applied Microbiology and Biotechnology·Kanae SakaiTakuya Nihira
May 24, 2012·Bioorganic & Medicinal Chemistry Letters·Beate WollinskyShu-Ming Li
Jun 12, 2012·Journal of Molecular Biology·Jan Michael SchullerThilo Stehle
Oct 5, 2012·Methods in Enzymology·Xia Yu, Shu-Ming Li
Oct 31, 2012·Chembiochem : a European Journal of Chemical Biology·Kathrin MundtShu-Ming Li
Nov 28, 2012·Nature Reviews. Microbiology·Axel A Brakhage
Aug 21, 2013·Applied Microbiology and Biotechnology·Binbin GuLixin Zhang

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Citations

Aug 1, 2015·Applied Microbiology and Biotechnology·Aili FanShu-Ming Li
Aug 19, 2015·Frontiers in Microbiology·Tobias W Giessen, Mohamed A Marahiel
Aug 19, 2015·Frontiers in Microbiology·Derek J MatternAxel A Brakhage
Sep 14, 2019·Biotechnology Advances·Antoine VassauxValérie Leclère
Oct 25, 2017·Journal of Natural Products·Kirsten Brockmeyer, Shu-Ming Li

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