Targeted projection NMR spectroscopy for unambiguous metabolic profiling of complex mixtures

Magnetic Resonance in Chemistry : MRC
Clément PontoizeauLyndon Emsley

Abstract

Unambiguous identification of individual metabolites present in complex mixtures such as biofluids constitutes a crucial prerequisite for quantitative metabolomics, toward better understanding of biochemical processes in living systems. Increasing the dimensionality of a given NMR correlation experiment is the natural solution for resolving spectral overlap. However, in the context of metabolites, natural abundance acquisition of (1)H and (13)C NMR data virtually excludes the use of higher dimensional NMR experiments (3D, 4D, etc.) that would require unrealistically long acquisition times. Here, we introduce projection NMR techniques for studies of complex mixtures, and we show how discrete sets of projection spectra from higher dimensional NMR experiments are obtained in a reasonable time frame, in order to capture essential information necessary to resolve assignment ambiguities caused by signal overlap in conventional 2D NMR spectra. We determine optimal projection angles where given metabolite resonances will have the least overlap, to obtain distinct metabolite assignment in complex mixtures. The method is demonstrated for a model mixture composition made of ornithine, putrescine and arginine for which acquisition of a sin...Continue Reading

References

Mar 1, 1995·Analytical Chemistry·J K NicholsonJ C Lindon
May 1, 1994·Journal of Biomolecular NMR·J P SimorreD Marion
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Apr 17, 1998·Journal of Magnetic Resonance·H BarjatA G Swanson
Sep 23, 1998·Trends in Biotechnology·S G OliverF Baganz
Mar 2, 2002·Science·Hiroaki Kitano
Jul 18, 2002·Nature Reviews. Drug Discovery·Jeremy K NicholsonElaine Holmes
Jan 30, 2003·Journal of the American Chemical Society·Seho Kim, Thomas Szyperski
May 20, 2004·Journal of the American Chemical Society·Eriks Kupce, Ray Freeman
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sebastian HillerGerhard Wider
Sep 8, 2005·Journal of the American Chemical Society·Hamid R EghbalniaJohn L Markley
May 4, 2006·Journal of the American Chemical Society·Eriks Kupce, Ray Freeman
May 30, 2006·Nature Medicine·Heide L KirschenlohrJames C Metcalfe
Mar 6, 2007·Angewandte Chemie·Fengli Zhang, Rafael Brüschweiler
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Narasimhamurthy ShanaiahDaniel Raftery
Aug 19, 2007·Journal of the American Chemical Society·Sebastian HillerKurt Wüthrich
Sep 12, 2007·Journal of the American Chemical Society·Ewen Lescop, Bernhard Brutscher
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Benjamin J BlaiseLyndon Emsley
Feb 13, 2008·The Journal of Chemical Physics·David A SnyderRafael Brüschweiler
May 31, 2008·Journal of Biomolecular NMR·Jochen VolkKurt Wüthrich
Jan 2, 2009·Analytical Chemistry·Patrick GiraudeauSerge Akoka
Jul 10, 2009·Analytical Chemistry·Benjamin J BlaisePierre Toulhoat
Oct 1, 2009·Magnetic Resonance in Chemistry : MRC·C LudwigU L Günther

❮ Previous
Next ❯

Citations

Aug 19, 2011·Analytical Chemistry·Kerem Bingol, Rafael Brüschweiler
Dec 13, 2012·Journal of Magnetic Resonance·Neil MacKinnonAyyalusamy Ramamoorthy
May 27, 2014·Analytical Chemistry·Adrien Le GuennecStefano Caldarelli
Nov 8, 2013·Analytical Chemistry·Kerem Bingol, Rafael Brüschweiler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.