Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response

Molecular Biology of the Cell
Yevgeniy V SerebrenikCraig M Crews

Abstract

In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regulated signaling. Golgi homeostasis mechanisms that regulate organelle structure and ensure precise processing and localization of protein substrates remain poorly understood. Using a chemical biology strategy to induce protein unfolding, we uncover a Golgi-specific transcriptional response. An RNA-sequencing profile of this stress response compared with the current state-of-the-art Golgi stressors, nigericin and xyloside, demonstrates the enhanced precision of Golgi targeting achieved with our system. The data set further reveals previously uncharacterized genes that we find to be essential for Golgi structural integrity. These findings highlight the Golgi's ability to sense misfolded proteins and establish new aspects of Golgi autoregulation.

References

May 7, 1990·Biochimica Et Biophysica Acta·H H MollenhauerL D Rowe
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·M MuñizA Velasco
Jul 29, 1998·Histochemistry and Cell Biology·A Dinter, E G Berger
Jun 20, 2000·The Journal of Biological Chemistry·M E MartinA Velasco
Jul 27, 2002·Annual Review of Cell and Developmental Biology·James Shorter, Graham Warren
Mar 27, 2003·Traffic·Ayano SatohGraham Warren
Sep 17, 2003·The Journal of Biological Chemistry·Frédéric BardPierre Jurdic
Jan 1, 1962·Acta Pathologica Et Microbiologica Scandinavica·F BIERRING
Oct 1, 2003·Molecular Biology of the Cell·Margarita CabreraAngel Velasco
Dec 4, 2004·The Journal of Biological Chemistry·Yanzhuang WangGraham Warren
Aug 24, 2006·Journal of Cell Science·Eloy BejaranoAngel Velasco
Mar 21, 2008·Nature Reviews. Molecular Cell Biology·Maria Antonietta De Matteis, Alberto Luini
Jun 7, 2008·ACS Chemical Biology·Georgyi V LosKeith V Wood
Jul 22, 2008·Nature Cell Biology·Teodoro PulvirentiAlberto Luini
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Jun 11, 2009·Seminars in Cell & Developmental Biology·Irene Barinaga-Rementeria Ramirez, Martin Lowe
Dec 17, 2009·Traffic·Fabiola MavillardAngel Velasco
Mar 3, 2010·Annual Review of Biophysics·Jennifer Lippincott-Schwartz, Robert D Phair
Jun 17, 2010·Methods in Molecular Biology·Hussam H Nour-EldinBarbara A Halkier
Dec 15, 2010·Cell Structure and Function·Masaya OkuHiderou Yoshida
Dec 29, 2010·Journal of Cell Science·Hesso Farhan, Catherine Rabouille
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Pamela Stanley
Apr 2, 2011·Cold Spring Harbor Perspectives in Biology·Peter Mayinger
Jul 5, 2011·Nature Chemical Biology·Taavi K NeklesaCraig M Crews
Jan 19, 2012·Nature Reviews. Molecular Cell Biology·Claudio Hetz
Jan 25, 2012·Chembiochem : a European Journal of Chemical Biology·Hyun Seop TaeCraig M Crews
Jun 23, 2012·Nature Reviews. Molecular Cell Biology·Kelley W MoremenAlison V Nairn
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
May 24, 2013·Nature Reviews. Molecular Cell Biology·Federica Brandizzi, Charles Barlowe
Aug 28, 2013·ACS Chemical Biology·Taavi K NeklesaCraig M Crews
Aug 31, 2013·Nature Reviews. Drug Discovery·Claudio HetzHeather P Harding
Sep 13, 2013·International Journal of Molecular Sciences·Mariana G Bexiga, Jeremy C Simpson
Mar 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gunjan JoshiYanzhuang Wang

❮ Previous
Next ❯

Citations

Jul 11, 2019·FEBS Letters·Kentaro Oh-HashiYoko Hirata
Jun 6, 2019·Molecular Biology of the Cell·Doris HellerschmiedCraig M Crews
Aug 6, 2019·FEBS Letters·Prajakta Kulkarni-GosaviPaul A Gleeson
May 20, 2020·Angewandte Chemie·Laura M LuhPhilipp M Cromm
Sep 23, 2018·Biochemical Society Transactions·Christian MakhoulPaul A Gleeson
Sep 16, 2020·The Journal of Experimental Medicine·Astrid HagelkruysJosef M Penninger
Jan 23, 2020·Nature Communications·Avital Eisenberg-LernerYifat Merbl
Jul 25, 2019·Frontiers in Cell and Developmental Biology·Christian MakhoulPaul A Gleeson
Jul 22, 2021·Cell Reports·Xin HeChristopher B Lawrence
Sep 10, 2021·BioFactors·Jiayin GaoLinxi Chen

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
glycosylation
confocal microscopy
transmission electron microscopy
PCR
electron microscopy
transfections
transfection
Assay
size-exclusion chromatography
column chromatography

Software Mentioned

iTEM
LightCycler 480
Tophat
Cufflinks
HOMER
Prism
GraphPad
house

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Science's STKE : Signal Transduction Knowledge Environment
Matthew W BunceRichard A Anderson
Proceedings of the National Academy of Sciences of the United States of America
Desiree A ThayerLily Yeh Jan
Cellular and Molecular Life Sciences : CMLS
R S Polishchuk, A A Mironov
© 2022 Meta ULC. All rights reserved