Targeted proteomics for quantification of histone acetylation in Alzheimer's disease.

Proteomics
Kangling ZhangWolff Kirsch

Abstract

The epigenetic remodeling of chromatin histone proteins by acetylation has been the subject of recent investigations searching for biomarkers indicative of late onset cognitive loss. Histone acetylations affect the regulation of gene transcription, and the loss of learning induced deacetylation at specific histone sites may represent biomarkers for memory loss and Alzheimer's disease (AD). Selected-reaction-monitoring (SRM) has recently been advanced to quantitate peptides and proteins in complex biological systems. In this paper, we provide evidence that SRM-based targeted proteomics can reliably quantify specific histone acetylations in both AD and control brain by identifying the patterns of H3 K18/K23 acetylations Results of targeted proteomics assays have been validated by Western blot (WB) analysis. As compared with LC-MS/MS-TMT (tandem-mass-tagging) and WB methods, the targeted proteomics method has shown higher throughput, and therefore promised to be more suitable for clinical applications. With this methodology, we find that histone acetylation is significantly lower in AD temporal lobe than found in aged controls. Targeted proteomics warrants increased application for studying epigenetics of neurodegenerative diseases.

References

Feb 21, 2007·Methods : a Companion to Methods in Enzymology·Amy R KnappMark R Parthun
Mar 13, 2009·Briefings in Functional Genomics & Proteomics·Anastasia K Yocum, Arul M Chinnaiyan
Mar 24, 2009·Analytical and Bioanalytical Chemistry·Luciano CallipoAldo Laganà
Jun 6, 2009·Expert Review of Proteomics·Kyunggon Kim, Youngsoo Kim
Jul 25, 2009·Journal of Alzheimer's Disease : JAD·Yitshak I FrancisOttavio Arancio
Aug 20, 2009·Analytical Chemistry·Tonya Pekar SecondVlad Zabrouskov
Sep 15, 2009·Biological Chemistry·Farahnaz Sananbenesi, Andre Fischer
Sep 22, 2009·Journal of Proteome Research·Mariana D Plazas-MayorcaBenjamin A Garcia
Oct 16, 2009·Molecular & Cellular Proteomics : MCP·Jesper V OlsenStevan Horning
Dec 17, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mark KilgoreGavin Rumbaugh
May 6, 2010·The Journal of Biological Chemistry·Agus DarwantoKangling Zhang
Sep 14, 2010·Nature Methods·Marko JovanovicMichael O Hengartner
Feb 22, 2011·The Journal of Biological Chemistry·Lei XiongKangling Zhang
Apr 7, 2011·Current Medicinal Chemistry·M DaniilidouM Tsolaki

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Citations

Sep 10, 2013·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Sivan Vadakkadath MeethalBermans J Iskandar
Jun 19, 2013·Journal of Proteome Research·Eric B DammerNicholas T Seyfried
Jun 27, 2014·Molecular Neurodegeneration·Carlos P FitzsimonsBart P F Rutten
Nov 26, 2013·Cardiovascular Research·Daliao XiaoLubo Zhang
Mar 26, 2014·Neuroscience Bulletin·Moses N WainainaChunjiu Zhong
Apr 23, 2014·Journal of Proteomics·Diogo Ribeiro DemartiniCélia Regina Carlini
Feb 19, 2013·Expert Review of Proteomics·Mithilesh Kumar Jha, Kyoungho Suk
May 9, 2014·International Journal of Molecular Sciences·Yahui LiuYulin Deng
Jul 6, 2014·Expert Opinion on Drug Discovery·Ramón Cacabelos, Clara Torrellas
Dec 26, 2015·International Journal of Molecular Sciences·Ramón Cacabelos, Clara Torrellas
Jan 7, 2016·Frontiers in Behavioral Neuroscience·Jose V Sanchez-Mut, Johannes Gräff
Nov 4, 2015·Frontiers in Behavioral Neuroscience·Xun Yu ChoongElizabeth M C Fisher
Sep 17, 2013·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Katie Lunnon, Jonathan Mill
Oct 16, 2015·Nature Reviews. Neuroscience·Hei-man Chow, Karl Herrup
Sep 12, 2015·Analytical Chemistry·James L SowersKangling Zhang
Jun 7, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·David A BennettPhilip L De Jager
Mar 17, 2015·Biochimica Et Biophysica Acta·Myoung-Goo KangYoung Mok Park
Aug 11, 2015·Mutation Research·Fabio Coppedè, Lucia Migliore
Jun 15, 2015·Progress in Neurobiology·Roy LardenoijeBart P F Rutten
Apr 3, 2014·Oxidative Medicine and Cellular Longevity·Yonggang WangLu Cai
Feb 18, 2015·Chemical Reviews·He HuangYingming Zhao
Jan 27, 2015·Biochimica Et Biophysica Acta·Ann BrinkmalmHenrik Zetterberg
Apr 30, 2013·Methods : a Companion to Methods in Enzymology·George E CraftAngus C Nairn
Jul 30, 2014·Frontiers in Genetics·Fabio Coppedè
Aug 31, 2014·Trends in Cell Biology·Bess FrostMel B Feany
Oct 16, 2014·Molecular & Cellular Proteomics : MCP·Dequina NicholasKangling Zhang
Mar 7, 2018·Nature Neuroscience·Raffaella NativioShelley L Berger
Jun 12, 2018·Neurodegenerative Disease Management·Andrea Stoccoro, Fabio Coppedè
Jun 6, 2018·Expert Review of Proteomics·Erica Gianazza, Cristina Banfi
Jan 14, 2018·Epigenetics & Chromatin·Sara El KennaniDelphine Pflieger

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