Jan 24, 2012

Targeted sequence capture as a powerful tool for evolutionary analysis

American Journal of Botany
Corrinne E GroverJ F Wendel

Abstract

Next-generation sequencing technologies (NGS) have revolutionized biological research by significantly increasing data generation while simultaneously decreasing the time to data output. For many ecologists and evolutionary biologists, the research opportunities afforded by NGS are substantial; even for taxa lacking genomic resources, large-scale genome-level questions can now be addressed, opening up many new avenues of research. While rapid and massive sequencing afforded by NGS increases the scope and scale of many research objectives, whole genome sequencing is often unwarranted and unnecessarily complex for specific research questions. Recently developed targeted sequence enrichment, coupled with NGS, represents a beneficial strategy for enhancing data generation to answer questions in ecology and evolutionary biology. This marriage of technologies offers researchers a simple method to isolate and analyze a few to hundreds, or even thousands, of genes or genomic regions from few to many samples in a relatively efficient and effective manner. These strategies can be applied to questions at both the infra- and interspecific levels, including those involving parentage, gene flow, divergence, phylogenetics, reticulate evolutio...Continue Reading

  • References95
  • Citations26

References

  • References95
  • Citations26

Mentioned in this Paper

Genes, Plant
Genome
Gene Expression Regulation, Plant
Phylogeny
Genomics
Isolate compound
Sequencing
Massively-Parallel Sequencing
Expressed Sequence Tags
Phylogeography

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