Targeted three-dimensional immunohistochemistry reveals localization of presynaptic proteins Bassoon and Piccolo in the rat calyx of Held before and after the onset of hearing

The Journal of Comparative Neurology
Anna DondzilloThomas Kuner

Abstract

Bassoon and Piccolo contribute to the cytomatrix of active zones (AZ), the sites of neurotransmitter release in nerve terminals. Here, we examined the 3D localization of Bassoon and Piccolo in the rat calyx of Held between postnatal days 9 and 21, the period of hearing onset characterized by pronounced structural and functional changes. Bassoon and Piccolo were identified by immunohistochemistry (IHC) on slices of the brainstem harboring calyces labeled with membrane-anchored green fluorescent protein (mGFP). By using confocal microscopy and 3D reconstructions, we examined the distribution of Bassoon and Piccolo in calyces delineated by mGFP. This allowed us to discriminate calyceal IHC signals from noncalyceal signals located in the spaces between the calyceal stalks, which could mimic a calyx-like distribution. We found that both proteins were arranged in clusters resembling the size of AZs. These clusters were located along the presynaptic membrane facing the principal cell, close to or overlapping with synaptic vesicle (SV) clusters. Only about 60% of Bassoon and Piccolo clusters overlapped, whereas the remaining clusters contained predominantly Bassoon or Piccolo, suggesting differential targeting of these proteins within ...Continue Reading

References

Dec 22, 1991·The Journal of Comparative Neurology·N KuwabaraJ M Zook
May 1, 1990·Journal of Neurophysiology·G A SpirouM Zidanic
Oct 1, 1989·Journal of Neurocytology·P Grandes, P Streit
Aug 22, 1989·The Journal of Comparative Neurology·A Schüz, G Palm
Nov 4, 1968·Zeitschrift Für Anatomie Und Entwicklungsgeschichte·D K Morest
Feb 8, 1993·The Journal of Comparative Neurology·K Kandler, E Friauf
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·T Schikorski, C F Stevens
May 26, 1999·The Journal of Comparative Neurology·K RichterE D Gundelfinger
Nov 17, 1999·The European Journal of Neuroscience·J H BrandstätterH Wässle
Aug 17, 2000·Biochemical and Biophysical Research Communications·L Sanmartí-VilaT Dresbach
Dec 24, 2002·Neuron·Holger TaschenbergerHenrique von Gersdorff
Jun 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dirk GrimmJuergen A Kleinschmidt
Jun 19, 2003·Molecular and Cellular Neurosciences·Thomas DresbachEckart D Gundelfinger
Dec 6, 2003·The European Journal of Neuroscience·Martine HamannIan D Forsythe
Mar 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gautam B AwatramaniLaurence O Trussell
Apr 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·S M WojcikC Rosenmund
Sep 29, 2004·Pflügers Archiv : European journal of physiology·Verena C WimmerThomas Kuner
Oct 1, 2004·Journal of Neurophysiology·Gautam B AwatramaniLaurence O Trussell
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Verena C WimmerThomas Kuner
Mar 16, 2006·The Journal of Comparative Neurology·Adrián Rodríguez-ContrerasJ Gerard G Borst
May 19, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brian K HoffpauirGeorge A Spirou
Jul 26, 2006·Cell and Tissue Research·Susanne Schoch, Eckart D Gundelfinger
Dec 13, 2006·The European Journal of Neuroscience·Judyta JuranekReinhard Jahn
Jun 29, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Léa SiksouSerge Marty
Jun 4, 2008·The Journal of Cell Biology·Sergio Leal-OrtizCraig C Garner
Jan 1, 2009·Nature Methods·Stefan W Hell

❮ Previous
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Citations

Sep 21, 2012·Molecular Neurobiology·Raquel CanoLucía Tabares
Nov 1, 2011·Annual Review of Physiology·J Gerard G Borst, John Soria van Hoeve
Jan 29, 2014·The Journal of Physiology·Giovanbattista GrandeLu-Yang Wang
Dec 21, 2012·Annals of the New York Academy of Sciences·Hiroshi Nishimune
Sep 22, 2011·The Journal of Comparative Neurology·Jie ChenHiroshi Nishimune
May 28, 2011·The Journal of Comparative Neurology·Samantha A SpanglerCasper C Hoogenraad
Jan 21, 2014·The EMBO Journal·Alejandro Mendoza SchulzTobias Moser
Jul 6, 2014·The European Journal of Neuroscience·Christoph KörberThomas Kuner
Apr 2, 2016·Progress in Neurobiology·Hans Gerd Nothwang
May 23, 2012·Cell Calcium·Raquel CanoWilliam J Betz
Feb 16, 2016·The Journal of Comparative Neurology·Eva K SawyerJon H Kaas
Nov 26, 2010·Neuron·Stefan HallermannJens Eilers
Jan 24, 2018·Journal of Neurophysiology·Rozita LaghaeiMarkus Dittrich
Dec 2, 2017·The Journal of Physiology·Daniel ParthierChristoph Körber
Nov 10, 2017·Frontiers in Synaptic Neuroscience·Tanvi ButolaTobias Moser
Nov 26, 2015·Nature Communications·Daniel Nunes, Thomas Kuner
Jan 14, 2021·The Journal of Physiology·Martijn C SierksmaJ Gerard G Borst
Jul 6, 2021·Frontiers in Synaptic Neuroscience·Kaarjel K NarayanasamyMike Heilemann
Nov 3, 2021·Physiological Reviews·Carolin Wichmann, Thomas Kuner

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