Targeting apoptosis by 1,2-diazole through regulation of EGFR, Bcl-2 and CDK-2 mediated signaling pathway in human non-small cell lung carcinoma A549 cells

Gene
Venugopal Vinod PrabhuKunnathur Murugesan Sakthivel

Abstract

Lung cancer is the leading cause of cancer deaths worldwide and non-small cell lung carcinoma (NSCLC), a heterogeneous class of tumors, represents approximately 85% of all new lung cancer diagnosis. Conventional treatment options have limited efficacy because most cases are in the advanced stage at the time of diagnosis. The present study evaluates the anti-cancer activity of 1,2-diazole (pyrazole), a natural compound from mangrove plant Rhizophora apiculata (R.apiculata) on A549 lung carcinoma cells. In the present study the anti-cancer mechanism of pyrazole, was examined by the expression level of proteins Epidermal growth factor receptor (EGFR), Bcl-2-associated X protein (Bax), B-cell lymphoma-2 (Bcl-2) and Cyclin-dependent kinase-2 (CDK-2) which are commonly associated with the cell signaling pathways that control cell survival and apoptosis, that could facilitate to develop a novel target and effective treatment approach for patients with NSCLC. Pyrazole significantly induced cell cycle arrest and initiated apoptosis through inhibition of downstream components of EGFR tyrosine kinase pathway. Pyrazole disrupts the mitochondrial membrane potential and modulated the protein levels of Bax and Bcl-2 which could probably lead ...Continue Reading

Citations

Mar 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nádia E SantosSusana Santos Braga
Oct 24, 2019·Journal of Hematology & Oncology·Yuan MaoQi Tang
Oct 21, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Alireza HabibiZeinab Moradi-Shoeili
Feb 19, 2021·Biotechnology Reports·Venugopal Vinod PrabhuKunnathur Murugesan Sakthivel
Aug 30, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Piao-Piao LiQiang Zhang

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