Targeting chemo-proton therapy on C6 cell line using superparamagnetic iron oxide nanoparticles conjugated with folate and paclitaxel

International Journal of Radiation Biology
Seong Hee KangBo Sun Kang

Abstract

This report presents novel nanoparticle-based drug delivery system (NPDDS) aiming to targeting chemo-proton therapy (TCPT) to improve the therapeutic efficacy on brain cancer treatments. A NPDDS, superparamagnetic iron oxide nanoparticles conjugated with folate and paclitaxel, was synthesized and applied to C6 brain cancer cell line that was prepared for TCPT. The characterization of NPDDS was analyzed by transmission electron microscope (TEM) and Fourier transform infrared (FTIR) spectroscopy. The uptake of NPDDS into the cytoplasm of C6 cells was observed by confocal laser scanning microscopy (CLSM). The therapeutic efficacy of proton beam was quantitatively evaluated by flow cytometry and clonogenic assay at various radiation dose. NPDDS was synthesized in the uniform size distribution with a mean diameter of 5.44 ± 0.70 nm, and it showed no significant cytotoxicity at the concentration lower than 200 ng/mL. Radiosensitization enhancement factors of PTX, D-SPIONs and FA-PTX-D-SPIONs were 1.35, 1.16 and 1.52, respectively. It was demonstrated that TCPT improved the therapeutic efficacy of the proton beam therapy when the synthesized novel NPDDS was administrated. The improvement in therapeutic efficacy was achieved by the syn...Continue Reading

References

Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D R Appling
Aug 4, 1983·The New England Journal of Medicine·R N KjellbergR D Adams
Aug 26, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R OrecchiaU Veronesi
Jun 17, 2000·Trends in Cell Biology·M J O'ConnellA M Carr
Sep 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Justin M SaulRavi V Bellamkonda
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Feb 3, 2006·International Journal of Cancer. Journal International Du Cancer·Linda E Kelemen
May 2, 2006·International Journal of Radiation Oncology, Biology, Physics·Rémi DendaleLaurence Desjardins
May 24, 2006·Cancer Chemotherapy and Pharmacology·Hongxia LuCongjian Xu
Jun 1, 2006·Journal of Biomedical Materials Research. Part a·Conroy SunMiqin Zhang
Jul 4, 2006·Seminars in Radiation Oncology·Patricia J Eifel
Jan 26, 2007·Pharmaceutical Research·Weijun Li, Francis C Szoka
Jul 11, 2008·Current Neuropharmacology·Charity D ScriptureAlex Sparreboom
May 14, 2010·Physics in Medicine and Biology·James F HainfeldHenry M Smilowitz
Feb 1, 2012·The Cancer Journal·Elizabeth R Gerstner, Tracy T Batchelor
Jul 7, 2012·Nanoscale·Karl T ButterworthKevin M Prise
Nov 15, 2012·Radiation Research·Ross I BerbecoG Mike Makrigiorgos
Feb 8, 2013·Medical Physics·Piotr ZygmanskiErno Sajo
May 28, 2013·International Journal of Radiation Oncology, Biology, Physics·Michael E WernerAndrew Z Wang
Jun 19, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Matthew LuchetteRoss Berbeco

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