Targeting Colorectal Cancer Cell Lines Using Nanobodies; AgSK1as a Potential Target

Iranian Journal of Biotechnology
Seyed Khalil RashidiWalead Ebrahimizadeh

Abstract

Background: Colorectal cancer is the third most common type of aggressive cancers. Chemotherapy, surgery, and radiotherapy are the common therapeutic options for treating this cancer. Due to the adverse side-effects of these methods, immunotherapy is considered as an appropriate alternative therapeutic option. Treatment through the application of monoclonal antibodies is considered as a novel alternative therapeutic method for cancers. The variable fragments of the antibodies' heavy chain or VHHs have a wide application in molecular biology and biotechnology. VHHs are compatible with the phage display technology which allows rapid and high throughput screening for antibodies isolation. Objectives: We aimed to use naive VHH phage library to isolate a specific nanobody against colorectal tumor associated antigen; the AgSK1. Materials and Methods: In this research, naive VHH phage library was panned against two colorectal cell lines; Ls174T and HT29 expressing different levels of AgSK1 tumor associated marker. The high affinity binders were selected and subcloned for higher expression levels of the VHH. The affinity and specificity of the isolated VHH were tested using ELISA. The reactivity of the VHH toward cancer cells was analy...Continue Reading

References

Jul 1, 1993·Japanese Journal of Cancer Research : Gann·T KudoA Kadota
Apr 17, 2004·Cancer Research·Virna Cortez-RetamozoHilde Revets
Aug 17, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Y A Luqmani
Jul 28, 2007·World Journal of Gastroenterology : WJG·Kathryn Field, Lara Lipton
Nov 6, 2007·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·J CapdevilaJ Tabernero
Nov 13, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hanna K SanoffRichard M Goldberg
Nov 26, 2008·Veterinary Immunology and Immunopathology·S MuyldermansD Saerens
Apr 21, 2009·Biotechnology Advances·Nina E Weisser, J Christopher Hall
Jan 14, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C BokemeyerP Koralewski
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Jun 19, 2012·Postȩpy higieny i medycyny doświadczalnej·Dorota SmolarekMarcin Czerwinski
Oct 30, 2012·Clinical Colorectal Cancer·Edith P Mitchell
Nov 9, 2012·Applied Microbiology and Biotechnology·Walead EbrahimizadehHamid Bakherad
Mar 20, 2013·Pharmacology & Therapeutics·Puja Sapra, Boris Shor
May 4, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yaqiong ZhouMin Wang
Jan 16, 2014·The International Journal of Biological Markers·Hamed ZareSeyed Latif Mousavi Gargari
Mar 29, 2014·Biotechnology and Applied Biochemistry·Roghayyeh BaghbanHamid Bakherad

❮ Previous
Next ❯

Citations

Aug 15, 2020·Frontiers in Oncology·Emily Y Yang, Khalid Shah
Nov 22, 2020·Life Sciences·Sepideh GhaniBahram Kazemi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

© 2022 Meta ULC. All rights reserved