Targeting cyclooxygenase-2 with sodium butyrate and NSAIDs on colorectal adenoma/carcinoma cells

World Journal of Gastroenterology : WJG
Zhi-Hong ZhangHua-Tian Gan

Abstract

The protective effects of sodium butyrate and NSAIDs (especially the highly selective COX-2 inhibitors) have attracted considerable interest recently. In this study, primary adenoma cells and HT-29 were used to investigate whether the above drugs would be effective for reducing proliferation and inducing apoptosis. Additionally, it was investigated whether NSAIDs would strengthen the effects of sodium butyrate and its possible mechanisms. In vitro primary cell culture of colorectal adenomas and HT-29 were used for this investigation. PGE2 isolated from HT-29 cell culture supernatants was investigated by ELISA. MTT was employed to detect the anti-proliferative effects on both adenoma and HT-29 culture cells. FCM was used for apoptosis rate and cell cycle analysis. The morphology of apoptotic cells was investigated by means of electromicroscopy. Sodium butyrate could stimulate the secretion of PGE2, while NSAIDs inhibited it to below 30 pg/10(6) cells. Both butyrate and NSAIDs could inhibit cell proliferation and induce apoptosis. The effects were time- and dose-dependent (P<0.05). Aspirin and NS-398 could enhance the effects of sodium butyrate. The effects were stronger while sodium butyrate was used in combination with NS-398 t...Continue Reading

References

Dec 13, 1996·Biochemical Pharmacology·W A Cromlish, B P Kennedy
Jan 1, 1997·Advances in Pharmacology·W E Smalley, R N DuBois
Apr 14, 1999·Cell Death and Differentiation·B BonnotteF Martin
May 11, 2002·The American Journal of Gastroenterology·Hong Zhang, Xiao-Feng Sun
May 23, 2002·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Christopher D RomanR Daniel Beauchamp
Jun 7, 2002·International Journal of Colorectal Disease·Manabu TakeuchiHitoshi Asakura
Jun 13, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wu-Guo DengKenneth K Wu
Jul 20, 2002·Veterinary Pathology·M F McEnteeN Neilsen

❮ Previous
Next ❯

Citations

Jul 12, 2008·Immunopharmacology and Immunotoxicology·Anna MahkonenPauli Puolakkainen
Jun 22, 2006·Journal of Cancer Research and Clinical Oncology·Mònica ComaladaJulio Gálvez
Jul 27, 2005·Biochemical Society Transactions·J M CoxheadJ C Mathers
Jun 7, 2005·Mechanisms of Ageing and Development·Kate M WebberMark A Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The American Journal of Gastroenterology
Shuichi SekiHiroaki Kinoshita
Nihon rinsho. Japanese journal of clinical medicine
S Kobayashi
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
Jian-ting GuanZong-guang Zhou
Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland
A GiatromanolakiE Sivridis
© 2021 Meta ULC. All rights reserved